Thomsen B M, Clausen H V, Larsen L G, Nürnberg L, Ottesen B, Thomsen H K
Department of Pathology, Bispebjerg Hospital, Denmark.
Placenta. 1997 Mar-Apr;18(2-3):145-54. doi: 10.1016/s0143-4004(97)90086-2.
The expression of insulin-like growth factor-II (IGF-II) in normal human first and third trimester placental tissue was investigated by non-isotopic in situ hybridization (ISH). This is the first ISH study on IGF-II expression in placenta using an alkaline phosphatase-labelled probe. The expression was correlated with the proliferative activity of the cells using the proliferative marker MIB-1. In first trimester tissue, IGF-II was expressed in the cytotrophoblast, the extravillous trophoblast, the fetal endothelial cells and the mesenchymal fetal cells in the villi. In third trimester tissue, IGF-II expression was found in the amnion, the extravillous trophoblast and the mesenchymal fetal cells especially in the endothelial cells and the outer contractile sheet in the stem villi. In areas with perivillous fibrin deposits, strong expression of IGF-II was found in the cytotrophoblasts invading the fibrin. In first trimester tissue, the proliferative activity of the villous cytotrophoblast correlated well with the degree of IGF-II expression whereas in third trimester tissue, there was a discrepancy between MIB-1 positivity and the IGF-II expression. Expression of IGF-II does not seem to be correlated exclusively to the mitogenic activity of cells.
采用非同位素原位杂交（ISH）技术研究了胰岛素样生长因子-II（IGF-II）在正常人类妊娠早期和晚期胎盘组织中的表达。这是首次使用碱性磷酸酶标记探针进行的关于胎盘组织中IGF-II表达的ISH研究。利用增殖标志物MIB-1将该表达与细胞的增殖活性进行关联。在妊娠早期组织中，IGF-II在细胞滋养层、绒毛外滋养层、胎儿内皮细胞和绒毛中的间充质胎儿细胞中表达。在妊娠晚期组织中，IGF-II表达见于羊膜、绒毛外滋养层和间充质胎儿细胞，尤其是在主干绒毛的内皮细胞和外层收缩层中。在有绒毛周围纤维蛋白沉积的区域，侵袭纤维蛋白的细胞滋养层中发现了强烈的IGF-II表达。在妊娠早期组织中，绒毛细胞滋养层的增殖活性与IGF-II表达程度密切相关，而在妊娠晚期组织中，MIB-1阳性与IGF-II表达之间存在差异。IGF-II的表达似乎并非仅与细胞的促有丝分裂活性相关。
