Phillips P, Voak D, Knowles S, Campbell G, Crane B, Downie D M, Haigh T, Whitton C
National Institute for Biological Standards and Control, Potters Bar, UK.
Transfus Med. 1997 Mar;7(1):47-53. doi: 10.1046/j.1365-3148.1997.d01-79.x.
Shear forces are proposed to explain the failure of antiglobulin and 'neutral' (no antiglobulin) microcolumn tests at 37 degrees C to detect weak ABO incompatibilities and other weak antibodies, clearly detectable by spin-tube methods. These shear forces can be minimized in a microcolumn test using a biphasic centrifugation phase. Although this biphasic test is not suitable for routine use, it may be of use as an investigational method for reference laboratories. This failure of microcolumn test to detect weak ABO incompatibilities is of little clinical significance as the antibodies are dubiously active at 37 degrees C.
有人提出，剪切力可解释抗球蛋白和“中性”（无抗球蛋白）微柱试验在37℃时无法检测出弱ABO血型不相容性及其他弱抗体的原因，而这些弱抗体用试管法却能清晰检测到。在微柱试验中采用双相离心阶段可将这些剪切力降至最低。尽管这种双相试验不适用于常规检测，但作为参考实验室的一种研究方法可能会有用。微柱试验未能检测出弱ABO血型不相容性在临床上意义不大，因为这些抗体在37℃时活性存疑。
