使用一种缺失E1基因、复制缺陷型腺病毒重组体，其表达狂犬病病毒糖蛋白，用于小鼠狂犬病病毒的早期疫苗接种。
The use of an E1-deleted, replication-defective adenovirus recombinant expressing the rabies virus glycoprotein for early vaccination of mice against rabies virus.
作者信息
Wang Y, Xiang Z, Pasquini S, Ertl H C
机构信息
Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
出版信息
J Virol. 1997 May;71(5):3677-83. doi: 10.1128/JVI.71.5.3677-3683.1997.
An E1-deleted, replication-defective adenovirus recombinant of the human strain 5 expressing the rabies virus glycoprotein, termed Adrab.gp, was tested in young mice. Mice immunized at birth with the Adrab.gp construct developed antibodies to rabies virus and cytokine-secreting lymphocytes and were protected against subsequent challenge. Maternal immunity to rabies virus strongly interferes with vaccination of the offspring with a traditional inactivated rabies virus vaccine. The immune response to the rabies virus glycoprotein, as presented by the Adrab.gp vaccine, on the other hand, was not impaired by maternal immunity. Even neonatal immunization of mice born to rabies virus-immune dams with Adrab.gp construct resulted in a long-lasting protective immune response to rabies virus, suggesting that this type of vaccine could be useful for immunization shortly after birth. Nevertheless, pups born to Adrab.gp virus-immune dams showed an impaired immune response to the rabies virus glycoprotein upon vaccination with the Adrab.gp virus, indicating that maternal immunity to the vaccine carrier affected the offspring's immune response to rabies virus.
一种表达狂犬病病毒糖蛋白的人5型E1缺失、复制缺陷型腺病毒重组体，称为Adrab.gp，在幼鼠中进行了测试。出生时用Adrab.gp构建体免疫的小鼠产生了针对狂犬病病毒的抗体和分泌细胞因子的淋巴细胞，并受到保护，免受随后的攻击。母体对狂犬病病毒的免疫力强烈干扰了用传统灭活狂犬病病毒疫苗对后代的接种。另一方面，Adrab.gp疫苗所呈现的对狂犬病病毒糖蛋白的免疫反应并未受到母体免疫力的损害。即使是用Adrab.gp构建体对狂犬病病毒免疫的母鼠所生的新生小鼠进行免疫，也会产生对狂犬病病毒的持久保护性免疫反应，这表明这种类型的疫苗可能对出生后不久的免疫有用。然而，Adrab.gp病毒免疫的母鼠所生的幼崽在用Adrab.gp病毒接种时，对狂犬病病毒糖蛋白的免疫反应受损，这表明母体对疫苗载体的免疫力影响了后代对狂犬病病毒的免疫反应。
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