核小体及核酸酶敏感DNA的甲基化

Methylation of nucleosomal and nuclease sensitive DNA.

作者信息

Adams R L, McKay E L, Douglas J T, Burdon R H

出版信息

Nucleic Acids Res. 1977 Sep;4(9):3097-108. doi: 10.1093/nar/4.9.3097.

DOI:10.1093/nar/4.9.3097

PMID:909800

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342637/

Abstract

The proportion of cytosines methylated in the DNA of nucleosome oligomers and of core particles appears indistinguishable from that of total nuclear DNA from CHO cells. However the DNA in nucleoprotein which is initially released from nuclei by treatment with very low levels of micrococcal nuclease and the first 10% of material rendered acid soluble by treatment of nuclei with DNase I are enriched 2 fold in their content of 5 methylcytosine. (Cessation of hydrolysis by nuclease occurs concomitantly with precipitation of nucleosomal core particles).

摘要

核小体寡聚体和核心颗粒DNA中甲基化胞嘧啶的比例似乎与CHO细胞核DNA的总比例没有区别。然而，通过用极低水平的微球菌核酸酶处理最初从细胞核中释放的核蛋白中的DNA，以及通过用DNase I处理细胞核使10%的物质变为酸溶性的DNA，其5-甲基胞嘧啶含量富集了2倍。（核酸酶水解的停止与核小体核心颗粒的沉淀同时发生）。