利用染色体涂染技术将马的U2连锁群定位到3号常染色体上。

Localization of the U2 linkage group of horses to ECA 3 using chromosome painting.

作者信息

Lear T L, Bailey E

机构信息

Department of Veterinary Science, M. H. Gluck Equine Research Center, University of Kentucky, Lexington 40546, USA.

出版信息

J Hered. 1997 Mar-Apr;88(2):162-4. doi: 10.1093/oxfordjournals.jhered.a023079.

DOI:10.1093/oxfordjournals.jhered.a023079

PMID:9099007

Abstract

The U2 linkage group of horses includes the genes albumin (ALB), vitamin D binding protein (GC), mitochondrial glutamate oxaloacetate transaminase 2 (GOT2), and haptoglobin (HP) which are found on two human chromosomes, namely, 4 (HSA 4) and 16 (HSA 16). Likewise these genes are also found on two different chromosomes in mice, rats, and cattle. Chromosome painting demonstrated that only horse chromosome 3 (ECA 3) hybridized with whole chromosome paints for both HSA 4 and HSA 16. This indicated that the equine U2 linkage group occurs on ECA 3, spanning the centromere. This technique will be useful to study the chromosome rearrangements associated with speciation of the genus Equus.

摘要

马的U2连锁群包含白蛋白（ALB）、维生素D结合蛋白（GC）、线粒体谷氨酸草酰乙酸转氨酶2（GOT2）和触珠蛋白（HP）等基因，这些基因在人类的两条染色体上被发现，即4号染色体（HSA 4）和16号染色体（HSA 16）。同样，这些基因在小鼠、大鼠和牛的两条不同染色体上也能找到。染色体涂染显示，只有马的3号染色体（ECA 3）能与HSA 4和HSA 16的全染色体涂染探针杂交。这表明马的U2连锁群位于ECA 3上，跨越着丝粒。这项技术将有助于研究与马属物种形成相关的染色体重排。