Raudsepp T, Otte K, Rozell B, Chowdhary B P
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, S-750 07 Uppsala, Sweden.
Mamm Genome. 1997 Aug;8(8):569-72. doi: 10.1007/s003359900505.
Three genomic subclones derived from a phage clone containing the equine IGF2 gene were used to FISH map the gene on horse (ECA) and donkey (EAS) metaphase chromosomes. The gene mapped on ECA 12q13 band and is the first locus mapped to this horse chromosome. In donkey the gene mapped very terminal on the long arm of one small submetacentric chromosome that shows almost identical DAPI-banding pattern with ECA12. This is the first locus mapped in donkey genome. Cross species chromosome painting of equine metaphase chromosomes with human Chromosome (Chr) 11-specific probe showed homoeology of this human chromosome with ECA12 and ECA7. The novel ECA12 comparative painting results are thus in accordance with the localization of the equine IGF2 gene. Comparison of the hitherto known physical locations of IGF2 in different species, viz. human, cattle, sheep, horse, and donkey, shows that this gene tends to maintain a terminal location on the chromosome arm.
从包含马IGF2基因的噬菌体克隆中获得的三个基因组亚克隆，用于在马（ECA）和驴（EAS）中期染色体上进行荧光原位杂交（FISH）定位该基因。该基因定位于ECA 12q13带，是第一个定位于这条马染色体上的基因座。在驴中，该基因定位于一条小亚中着丝粒染色体长臂的非常末端位置，该染色体显示出与ECA12几乎相同的DAPI带型。这是在驴基因组中定位的第一个基因座。用人染色体（Chr）11特异性探针进行马中期染色体的跨物种染色体涂染，显示该人类染色体与ECA12和ECA7具有同源性。因此，新的ECA12比较涂染结果与马IGF2基因的定位一致。比较迄今已知的IGF2在不同物种（即人类、牛、羊、马和驴）中的物理位置，表明该基因倾向于在染色体臂上保持末端位置。
