Bhattacharya R, Sugendran K, Dangi R S, Rao P V
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Biomed Environ Sci. 1997 Mar;10(1):93-101.
Nephrotoxic potential of laboratory cultures of freshwater cyanobacterium (blue-green alga) Microcystis aeruginosa PCC 7806 (Pasteur Institute) was assessed in male rats. The animals were injected intraperitoneally with 0.5, 1.0 and 2.0 LD50 doses of lyophilized cell extract. Elevated plasma urea and creatinine levels were accompanied by decrease in protein and albumin levels, followed by hematuria, proteinuria and bilirubinuria. Also decrease in kidney lactate dehydrogenase and glutamic oxaloacetic transaminase indicated possible nephrotoxic potential of the cyanobacteria. The extract also produced various hematological changes associated with stagnant type of hypoxia. High performance liquid chromatography of the culture identified the active principle (toxin) as Microcystin-LR.
在雄性大鼠中评估了淡水蓝藻（蓝绿藻）铜绿微囊藻PCC 7806（巴斯德研究所）实验室培养物的肾毒性潜力。给动物腹腔注射0.5、1.0和2.0 LD50剂量的冻干细胞提取物。血浆尿素和肌酐水平升高，同时蛋白质和白蛋白水平降低，随后出现血尿、蛋白尿和胆红素尿。肾脏乳酸脱氢酶和谷草转氨酶降低也表明蓝藻可能具有肾毒性潜力。提取物还产生了与淤滞型缺氧相关的各种血液学变化。对培养物进行高效液相色谱分析确定活性成分（毒素）为微囊藻毒素-LR。
