Navarro X, Verdú E, Wendelschafer-Crabb G, Kennedy W R
Department of Cell Biology and Physiology, Universitat Autònoma de Barcelona, Bellaterra, Spain.
J Comp Neurol. 1997 Apr 7;380(2):164-74. doi: 10.1002/(sici)1096-9861(19970407)380:2<164::aid-cne2>3.0.co;2-1.
The time sequence of sensory and sudomotor nerve regeneration to the mouse footpad was studied between one and seven weeks after crush or section of the sciatic nerve. Protein gene product 9.5, vasoactive intestinal peptide, substance P, and calcitonin gene-related peptide were localized in thick sections by using indirect immunofluorescence techniques and imaged by confocal microscopy. Nerve regeneration was visually assessed in all nerves and quantified in sweat glands. After denervation, protein gene product 9.5 immunoreactivity remained as dim fluorescence within thick fibers of dermal nerve trunks, whereas thin nerve fibers to sweat glands and to epidermis disappeared. By 14 days postcrush and 35 days postsection, the first protein gene product 9.5 immunoreactive regenerating axons appeared in large nerve trunks, quickly extending to epidermis and sweat glands. Reinnervation of Meissner's corpuscles occurred nearly simultaneous with return of epidermal free nerve endings and sudomotor network. Calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P immunoreactivity disappeared completely one week after denervation, then reappeared at 17-18 days postcrush and 35 days postsection. Fewer nerve fibers were immunoreactive to these peptides than to protein gene product 9.5. The overall density of reinnervation, although reduced, more closely resembled normal in the sweat glands and Meissner's corpuscles than in the epidermis. Reinnervation was more successful after crush than after nerve section. The time course for functional return of sweating paralleled the return of protein gene product 9.5 immunoreactivity, whereas appearance of vasoactive intestinal peptide was delayed by several days.
在坐骨神经挤压或切断后的1至7周内，研究了小鼠足垫感觉神经和汗腺运动神经再生的时间顺序。通过间接免疫荧光技术在厚切片中定位蛋白质基因产物9.5、血管活性肠肽、P物质和降钙素基因相关肽，并通过共聚焦显微镜成像。对所有神经的神经再生进行视觉评估，并对汗腺进行定量分析。去神经支配后，蛋白质基因产物9.5免疫反应性在真皮神经干的粗纤维中保留为暗淡荧光，而支配汗腺和表皮的细神经纤维消失。挤压后14天和切断后35天，第一批蛋白质基因产物9.5免疫反应性再生轴突出现在大神经干中，并迅速延伸至表皮和汗腺。迈斯纳小体的神经再支配几乎与表皮游离神经末梢和汗腺运动网络的恢复同时发生。降钙素基因相关肽、血管活性肠肽和P物质免疫反应性在去神经支配1周后完全消失，然后在挤压后17 - 18天和切断后35天重新出现。与蛋白质基因产物9.5相比，对这些肽免疫反应的神经纤维较少。尽管神经再支配的总体密度降低，但在汗腺和迈斯纳小体中比在表皮中更接近正常情况。挤压后神经再支配比切断后更成功。出汗功能恢复的时间进程与蛋白质基因产物9.5免疫反应性的恢复平行，而血管活性肠肽的出现则延迟了几天。
