Soldatov A V, Nabirochkina E N
Genetika. 1996 Dec;32(12):1717-20.
A new method for cloning Drosophila melanogaster genes genetically localized and marked with the insert of a highly copied mobile element is described. A clue step of the method involves a genetic scheme that allows two series of D. melanogaster strains differing only in genomic location of the studied gene to be obtained. This method allowed the e(y)1 gene marked with an insert of the Stalker highly copied transposon to be cloned [1].
本文描述了一种克隆果蝇基因的新方法，这些基因在遗传上已定位，并通过高拷贝移动元件的插入进行了标记。该方法的一个关键步骤涉及一种遗传方案，该方案可获得仅在所研究基因的基因组位置上不同的两个系列的黑腹果蝇菌株。利用这种方法，已克隆出用Stalker高拷贝转座子插入标记的e(y)1基因[1]。
