Corpet D E, Taché S, Peiffer G
Ecole Nationale Vétérinaire, Laboratoire de Sécurité des Aliments, Institut National de la Recherche Agronomique, Toulouse, France.
Cancer Lett. 1997 Mar 19;114(1-2):135-8. doi: 10.1016/s0304-3835(97)04643-0.
Promotion would suppose the selection of initiated cells. We tested the selection of aberrant crypt cells by cholic acid, a colon cancer promoter, and the effect of protectors, phytate and food restriction. After an azoxymethane injection, rats were allocated to a control diet, or to supplements of cholic acid, sodium phytate, or to a 50% food restriction. The proliferation and apoptosis of 1200 crypts were assessed, after immuno-staining for BrdU. Cholic acid increased the proliferation of aberrant crypts but not of normal crypts. Phytate and food restriction decreased the proliferation of normal crypts, but not of aberrant crypts. Apoptosis was not affected by diets. Results support the hypothesis that cholic acid can select initiated cells in the colon.
促进作用可能意味着对起始细胞的选择。我们测试了结肠癌促进剂胆酸对异常隐窝细胞的选择作用，以及肌醇六磷酸和食物限制等保护剂的影响。注射偶氮甲烷后，将大鼠分为对照组饮食，或补充胆酸、肌醇六磷酸钠组，或进行50%的食物限制组。在对BrdU进行免疫染色后，评估了1200个隐窝的增殖和凋亡情况。胆酸增加了异常隐窝的增殖，但未增加正常隐窝的增殖。肌醇六磷酸和食物限制降低了正常隐窝的增殖，但未降低异常隐窝的增殖。饮食对凋亡没有影响。结果支持胆酸可以在结肠中选择起始细胞这一假说。
