Roncucci L, Pedroni M, Vaccina F, Benatti P, Marzona L, De Pol A
Department of Internal Medicine, University of Modena, Italy.
Cell Prolif. 2000 Feb;33(1):1-18. doi: 10.1046/j.1365-2184.2000.00159.x.
Aberrant crypt foci (ACF) have been identified on the colonic mucosal surface of rodents treated with colon carcinogens and of humans after methylene-blue staining and observation under a light microscope. Several lines of evidence strongly suggest that ACF with certain morphological, histological, cell kinetics, and genetic features are precursor lesions of colon cancer both in rodents and in humans. Thus, ACF represent the earliest step in colorectal carcinogenesis. This paper has the main purpose of reviewing the evidence supporting this view, with particular emphasis on cell and crypt dynamics in ACF. ACF have been used as intermediate biomarkers of cancer development in animal studies aimed at the identification of colon carcinogens and chemopreventive agents. Recently, evidence has also shown that ACF can be effectively employed in chemopreventive studies also in humans.
在用结肠癌致癌剂处理过的啮齿动物以及经亚甲蓝染色并在光学显微镜下观察的人类结肠黏膜表面,已发现异常隐窝灶(ACF)。多条证据有力地表明,具有某些形态学、组织学、细胞动力学和遗传学特征的ACF在啮齿动物和人类中都是结肠癌的前体病变。因此,ACF代表了结直肠癌发生的最早阶段。本文的主要目的是回顾支持这一观点的证据,特别强调ACF中的细胞和隐窝动态。在旨在鉴定结肠癌致癌剂和化学预防剂的动物研究中,ACF已被用作癌症发展的中间生物标志物。最近,证据还表明ACF在人类化学预防研究中也可有效应用。