Shimizu S, Ogawa J, Kataoka M, Kobayashi M
Department of Agricultural Chemistry, Kyoto University, Japan.
Adv Biochem Eng Biotechnol. 1997;58:45-87. doi: 10.1007/BFb0103302.
Enzymes have been generally accepted as superior catalysts in organic synthesis. Micro-organisms in particular have been regarded as treasure sources of useful enzymes. The synthetic technology using microbial enzymes or micro-organisms themselves is called microbial transformation. In designing a microbial transformation process, one of the most important points is to find a suitable enzyme for the reaction of interest. Various kinds of novel enzymes for specific transformations have been discovered in micro-organisms and their potential characteristics revealed. This article reviews our current results on the discovery of novel enzymes for the production of biologically and chemically useful compounds, and emphasizes the importance of screening enzymes in a diverse microbial world.
酶已被公认为有机合成中卓越的催化剂。特别是微生物，一直被视为有用酶的宝库。利用微生物酶或微生物本身的合成技术称为微生物转化。在设计微生物转化过程时，最重要的一点是找到一种适合目标反应的酶。在微生物中发现了各种用于特定转化的新型酶，并揭示了它们的潜在特性。本文综述了我们目前在发现用于生产生物和化学有用化合物的新型酶方面的成果，并强调了在多样的微生物世界中筛选酶的重要性。
