Henderson I R, Meehan M, Owen P
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Ireland.
FEMS Microbiol Lett. 1997 Apr 1;149(1):115-20. doi: 10.1111/j.1574-6968.1997.tb10317.x.
The product controlling colony form variation and autoaggregation in Escherichia coli K-12 (the flu gene product) has been identified as the phase-variable, bipartite, outer membrane protein, termed antigen 43 (Ag43). Identification is based: (i) on complete correlation in authentic flu variants between colony morphology/autoaggregation and Ag43 expression as determined by colony and Western immunoblotting and immunofluorescence microscopy; and (ii) on the use of a specific probe to map the gene encoding Ag43 to a position (min 43) on the E. coli chromosome previously established for flu.
在大肠杆菌K-12中控制菌落形态变异和自聚集的产物（流感基因产物）已被鉴定为相变可变的二分体外膜蛋白，称为抗原43（Ag43）。鉴定的依据是：（i）通过菌落、Western免疫印迹和免疫荧光显微镜测定，在真实的流感变体中，菌落形态/自聚集与Ag43表达完全相关；（ii）使用特异性探针将编码Ag43的基因定位到大肠杆菌染色体上先前确定的流感基因所在位置（43分钟处）。
