Szczebara F, Dhaenens L, Vincent P, Husson M O
Laboratoire de Bactériologie-Hygiène, Faculté de médecine, Lille, France.
Eur J Clin Microbiol Infect Dis. 1997 Feb;16(2):162-4. doi: 10.1007/BF01709478.
Resistance of Helicobacter pylori to clarithromycin is due to point mutations at position A2143 or A2144 of the rrnH 23S rRNA gene, each mutation creating an additional restriction site for BsaI or MboII. A procedure combining PCR and RFLP analysis was evaluated for detection of these mutations using primers specific for the 23S rRNA gene, and BsaI and MboII enzymes. All clarithromycin-resistant isolates (8/8), as defined by the MIC, were found to be resistant by PCR-RFLP. No clarithromycin-sensitive isolates (14/14) gave a positive reaction.
幽门螺杆菌对克拉霉素的耐药性是由于rrnH 23S rRNA基因的A2143或A2144位点发生点突变，每个突变都会为BsaI或MboII产生一个额外的限制性酶切位点。使用针对23S rRNA基因的特异性引物以及BsaI和MboII酶，对一种结合PCR和RFLP分析的方法进行了评估，以检测这些突变。所有根据最低抑菌浓度定义的克拉霉素耐药菌株（8/8）通过PCR-RFLP检测均显示耐药。没有克拉霉素敏感菌株（14/14）出现阳性反应。
