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本文引用的文献

1
One stomach--one strain: does Helicobacter pylori strain variation influence disease outcome?一个胃部——一种菌株：幽门螺杆菌菌株变异会影响疾病结局吗？
Dig Dis Sci. 1999 Jan;44(1):102-7. doi: 10.1023/a:1026658301825.
2
Identification of a 23S rRNA gene mutation in clarithromycin-resistant Helicobacter pylori.耐克拉霉素幽门螺杆菌中23S rRNA基因突变的鉴定
Helicobacter. 1996 Dec;1(4):227-8. doi: 10.1111/j.1523-5378.1996.tb00043.x.
3
Differentiation of Helicobacter pylori strains directly from gastric biopsy specimens by PCR-based restriction fragment length polymorphism analysis without culture.无需培养，通过基于聚合酶链反应的限制性片段长度多态性分析直接从胃活检标本中鉴别幽门螺杆菌菌株。
J Clin Microbiol. 1997 Dec;35(12):3021-5. doi: 10.1128/jcm.35.12.3021-3025.1997.
4
Treatment of Helicobacter pylori infection: a review of the world literature.幽门螺杆菌感染的治疗：世界文献综述
Helicobacter. 1996 Mar;1(1):6-19. doi: 10.1111/j.1523-5378.1996.tb00003.x.
5
Eradication of Helicobacter pylori using one-week triple therapies combining omeprazole with two antimicrobials: the MACH I Study.使用奥美拉唑与两种抗菌药物联合的一周三联疗法根除幽门螺杆菌：MACH I研究
Helicobacter. 1996 Sep;1(3):138-44. doi: 10.1111/j.1523-5378.1996.tb00027.x.
6
Helicobacter pylori infection in the pathogenesis of duodenal ulcer and gastric cancer: a model.幽门螺杆菌感染在十二指肠溃疡和胃癌发病机制中的作用：一种模型。
Gastroenterology. 1997 Dec;113(6):1983-91. doi: 10.1016/s0016-5085(97)70019-2.
7
Macrolide resistance in Helicobacter pylori: mechanism and stability in strains from clarithromycin-treated patients.幽门螺杆菌对大环内酯类药物的耐药性：来自克拉霉素治疗患者菌株的机制与稳定性
Antimicrob Agents Chemother. 1997 Nov;41(11):2550-3. doi: 10.1128/AAC.41.11.2550.
8
Current European concepts in the management of Helicobacter pylori infection. The Maastricht Consensus Report. European Helicobacter Pylori Study Group.欧洲幽门螺杆菌感染管理的当前概念。马斯特里赫特共识报告。欧洲幽门螺杆菌研究小组。
Gut. 1997 Jul;41(1):8-13. doi: 10.1136/gut.41.1.8.
9
Evaluation of rapid molecular methods for detection of clarithromycin resistance in Helicobacter pylori.用于检测幽门螺杆菌对克拉霉素耐药性的快速分子方法的评估
Eur J Clin Microbiol Infect Dis. 1997 Feb;16(2):162-4. doi: 10.1007/BF01709478.
10
A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori.一种用于测定克拉霉素耐药幽门螺杆菌中23S rRNA基因突变发生率的聚合酶链反应-寡核苷酸连接测定法。
Antimicrob Agents Chemother. 1997 Mar;41(3):712-4. doi: 10.1128/AAC.41.3.712.

直接从胃活检材料中快速PCR检测幽门螺杆菌相关毒力和耐药基因。

Rapid PCR detection of Helicobacter pylori-associated virulence and resistance genes directly from gastric biopsy material.

作者信息

Björkholm B, Befrits R, Jaup B, Engstrand L

机构信息

Department of Medicine, Karolinska Institute, Stockholm, Sweden.

出版信息

J Clin Microbiol. 1998 Dec;36(12):3689-90. doi: 10.1128/JCM.36.12.3689-3690.1998.

DOI:10.1128/JCM.36.12.3689-3690.1998
PMID:9817898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105265/
Abstract

We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.

摘要

我们开发了一种基于聚合酶链反应（PCR）的方法，可在24小时内检测幽门螺杆菌对大环内酯类药物的耐药性以及毒力基因cagA，从而改进了基于培养方法的漫长流程。直接从胃活检标本中提取总DNA。该方法被证明快速可靠，可用于诊断目的。