直接从胃活检材料中快速PCR检测幽门螺杆菌相关毒力和耐药基因。
Rapid PCR detection of Helicobacter pylori-associated virulence and resistance genes directly from gastric biopsy material.
作者信息
Björkholm B, Befrits R, Jaup B, Engstrand L
机构信息
Department of Medicine, Karolinska Institute, Stockholm, Sweden.
出版信息
J Clin Microbiol. 1998 Dec;36(12):3689-90. doi: 10.1128/JCM.36.12.3689-3690.1998.
Abstract
We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.
摘要
我们开发了一种基于聚合酶链反应（PCR）的方法，可在24小时内检测幽门螺杆菌对大环内酯类药物的耐药性以及毒力基因cagA，从而改进了基于培养方法的漫长流程。直接从胃活检标本中提取总DNA。该方法被证明快速可靠，可用于诊断目的。
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