Jorgensen P F, Hyldgaard-Jensen J, Moustgaard J
Acta Vet Scand. 1977;18(3):323-34. doi: 10.1186/BF03548430.
Determination of the seleno-enzyme glutathione peroxidase (GSH-Px) in blood from Danish Landrace pigs was done using a quantitative, spectrophotometric method and a simple “spot test”. A close correlation between the net reaction rate measured spectrophotometrically (Δ A/min.) and time for defluores-cence (minutes) was obtained (r = 0.72—0.77, P < 0.0005). From these results the factors used for a conversion of defluorescence time to u/g hemoglobin were evaluated. The results further showed that the “spot test” can be used as a screening method for detection of subnormal GSH-Px levels in pigs. While red cell GSH-Px seems independent of the sex, an elevation of both plasma and red cell GSH-Px was found with increasing age of pigs. The normal range of red cell GSH-Px activity was wide, contrasting the small variations observed in the individual pig. Some evidence that porcine red cell GSH-Px is under genetical control was found and discussed in relation to the possible use of GSH-Px as an indicator of the pig's selenium status.
采用定量分光光度法和一种简单的“斑点试验”对丹麦长白猪血液中的硒酶谷胱甘肽过氧化物酶（GSH-Px）进行了测定。通过分光光度法测得的净反应速率（ΔA/分钟）与荧光消退时间（分钟）之间呈现出密切的相关性（r = 0.72 - 0.77，P < 0.0005）。基于这些结果，对用于将荧光消退时间转换为每克血红蛋白含酶量（u/g血红蛋白）的因子进行了评估。结果还表明，“斑点试验”可作为一种筛选方法，用于检测猪体内GSH-Px水平低于正常的情况。虽然红细胞GSH-Px似乎与性别无关，但随着猪年龄的增长，血浆和红细胞中的GSH-Px均有所升高。红细胞GSH-Px活性的正常范围较宽，这与个体猪所观察到的微小变化形成对比。发现了一些表明猪红细胞GSH-Px受遗传控制的证据，并就将GSH-Px用作猪硒状态指标的可能性进行了讨论。
