Dalsgaard K, Jensen M H, Sorensen K J
Acta Vet Scand. 1977;18(3):349-60. doi: 10.1186/BF03548432.
The saponin adjuvant Quil A has been investigated in the vaccination of cattle against foot-and-mouth disease. Using a Frenkel type vaccine a dose-response relationship has been established between Quil A and neutralizing antibody titres. Ten ml of vaccine was combined with 0, 50, 200, 800, and 3200 µg of Quil A. The combinations were each injected into 4 animals. The local reaction on the site of injection produced by injection of the vaccine alone and in combination with different doses of Quil A has been estimated. On this basis a therapeutical dose at 1 mg of Quil A has been estimated to combine maximum adjuvant effect with a minimum of adverse reactions. This dose has been tested in the vaccination of cattle with FMD vaccines derived from BHK suspension cell virus of type O and A respectively. The vaccines were tested in 10 ml and 5 ml doses with or without Quil A, and each in 4 animals. It is concluded that Quil A is a valuable adjuvant for use in the induction of neutralizing antibodies against foot-and-mouth disease in cattle.
皂苷佐剂Quil A已在牛口蹄疫疫苗接种中进行了研究。使用弗伦克尔型疫苗，已确定Quil A与中和抗体滴度之间的剂量反应关系。将10毫升疫苗与0、50、200、800和3200微克的Quil A混合。每种组合分别注射到4只动物体内。已评估了单独注射疫苗以及与不同剂量Quil A联合注射在注射部位产生的局部反应。在此基础上，估计1毫克Quil A的治疗剂量可将最大佐剂效果与最小不良反应相结合。该剂量已在分别用源自O型和A型BHK悬浮细胞病毒的口蹄疫疫苗对牛进行的疫苗接种中进行了测试。疫苗分别以10毫升和5毫升剂量进行测试，有或没有Quil A，每种剂量在4只动物中进行测试。得出的结论是，Quil A是一种有价值的佐剂，可用于诱导牛产生针对口蹄疫的中和抗体。
