Dalsgaard K, Jensen M H
Acta Vet Scand. 1977;18(3):367-73. doi: 10.1186/BF03548434.
The saponin adjuvant Quil A was investigated in trivalent vaccination against foot-and-mouth disease with a concentrated vaccine based on BHK suspension cell virus of the serotypes O, A and G. The activity in cattle was estimated on the basis of seroneutra-lizing antibodies. Five and 10 ml doses with or without 1 mg of Quil A were each injected into 6 animals. Seroneutralizing antibodies were estimated at regular intervals during a period of 29 weeks. The activity in guinea pigs was estimated by experimental challenge. One ml doses of serial 4-fold dilutions of the vaccine with or without 50 µg of Quil A were injected into 24 groups of 20 guinea pigs. Challenge was given 3 weeks after vaccination. It was concluded that Quil A showed adjuvant activity in cattle and guinea pigs with all the serotypes used in the trivalent vaccination.
使用基于O、A和G型血清型的BHK悬浮细胞病毒的浓缩疫苗，在三价口蹄疫疫苗接种中研究了皂苷佐剂Quil A。基于血清中和抗体评估其在牛体内的活性。将含或不含1毫克Quil A的5毫升和10毫升剂量分别注射到6只动物体内。在29周的时间内定期评估血清中和抗体。通过实验性攻毒评估其在豚鼠体内的活性。将含或不含50微克Quil A的疫苗系列4倍稀释液的1毫升剂量注射到24组每组20只豚鼠体内。接种疫苗3周后进行攻毒。得出的结论是，Quil A在三价疫苗接种中使用的所有血清型的牛和豚鼠中均显示出佐剂活性。
