Lou J, Chofflon M, Juillard C, Donati Y, Mili N, Siegrist C A, Grau G E
Department of Anaesthesiology, Pharmacology, and Surgical Intensive Care, Hôpital Cantonal Universitaire, Centre Médical Universitaire (CMU), University of Geneva, Switzerland.
Neuroreport. 1997 Feb 10;8(3):629-33. doi: 10.1097/00001756-199702100-00010.
The adhesion properties of brain microvascular endothelial cells (MVEC) and leukocytes derived from patients with multiple sclerosis (MS) were investigated. Leukocytes and brain MVEC from MS patients exhibited significantly higher adhesion capacity than the same cells isolated from normal donors. Flow cytometry showed that MS-derived brain MVEC constitutively expressed higher levels of ICAM-1 and contained an increased proportion of MHC class II positive cells than normal brain MVEC. In contrast, no difference was seen for vascular cell adhesion molecule-1 and endothelial cell leukocyte adhesion molecule-1. Circulating leukocytes from MS patients expressed higher levels of LFA-1, a ligand of intercellular adhesion molecule-1 (ICAM-1), than did normal leukocytes. The data presented here suggest that the ICAM-1/LFA-1 interaction may determine cytoadherence of leukocytes to brain MVEC in MS.
对多发性硬化症（MS）患者来源的脑微血管内皮细胞（MVEC）和白细胞的黏附特性进行了研究。MS患者的白细胞和脑MVEC比从正常供体分离出的相同细胞表现出显著更高的黏附能力。流式细胞术显示，与正常脑MVEC相比，MS来源的脑MVEC组成性地表达更高水平的细胞间黏附分子-1（ICAM-1），并且MHC II类阳性细胞的比例增加。相比之下，血管细胞黏附分子-1和内皮细胞白细胞黏附分子-1没有差异。MS患者的循环白细胞比正常白细胞表达更高水平的淋巴细胞功能相关抗原-1（LFA-1），后者是细胞间黏附分子-1（ICAM-1）的配体。此处呈现的数据表明，ICAM-1/LFA-1相互作用可能决定MS中白细胞与脑MVEC的细胞黏附。
