Furihata C, Oka M, Yamamoto M, Ito T, Ichinose M, Miki K, Tatematsu M, Sakaki Y, Reske K
Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Japan.
Cancer Res. 1997 Apr 15;57(8):1416-8.
Administration of N-methyl-N'-nitro-N-nitrosoguanidine, a glandular stomach carcinogen, at the concentration of 100 microg/ml in drinking water for 8 days induced the appearance of a MHC class II-associated invariant chain in the target organ of stomach pyloric mucosa of male Lewis rats. The up-regulation of the MHC class II-associated invariant chain was revealed by fluorescent differential display analysis, reverse transcription-PCR, Northern blot, and histochemical staining. The appearance of MHC class II and MHC class I was also demonstrated by reverse transcription-PCR and Northern blot. The results suggest the involvement of MHC-controlled immune reactions in chemically-induced stomach carcinogenesis.
以100微克/毫升的浓度在饮用水中给予雄性Lewis大鼠胃幽门黏膜的靶器官N-甲基-N'-硝基-N-亚硝基胍（一种腺胃致癌物）8天，可诱导MHC II类相关恒定链的出现。通过荧光差异显示分析、逆转录聚合酶链反应、Northern印迹和组织化学染色揭示了MHC II类相关恒定链的上调。逆转录聚合酶链反应和Northern印迹也证实了MHC II类和MHC I类的出现。结果表明MHC控制的免疫反应参与了化学诱导的胃癌发生。
