Rifai N, Sakamoto M, Lafi M, Guinan E
Department of Laboratory Medicine, Children's Hospital, Boston, MA 02115, USA.
Ther Drug Monit. 1997 Apr;19(2):169-74. doi: 10.1097/00007691-199704000-00009.
Busulfan is widely used in bone marrow transplantation. Increased area under the plasma concentration curve has been shown to correlate with venoocclusive disease, which occurs in approximately 20% of these patients. The authors developed a high performance liquid chromatography assay for the determination of plasma busulfan concentration using ultraviolet detection and a single-step derivatization and extraction. The absolute retention times of busulfan and the internal standard were 2.8 and 5.6 min, respectively. The assay possessed linearity up to 200 mumol/l, sensitivity to at least 0.2 mumol/l, average recovery of 101%, and run-to-run precision (n = 34) of < 7%. Furthermore, the assay proved to be free of interference from 59 medications and correlated highly with a validated method employing gas chromatography with electron capture detection (slope = 0.90, intercept = 0.17, r = 0.98, n = 31). The authors conclude that the method described here is ideally suited for the therapeutic monitoring of busulfan.
白消安广泛应用于骨髓移植。血浆浓度曲线下面积增加已被证明与静脉闭塞性疾病相关，约20%的此类患者会发生该疾病。作者开发了一种高效液相色谱法，用于测定血浆白消安浓度，采用紫外检测以及单步衍生化和萃取。白消安和内标的绝对保留时间分别为2.8分钟和5.6分钟。该测定法线性范围高达200μmol/L，灵敏度至少为0.2μmol/L，平均回收率为101%，批间精密度（n = 34）<7%。此外，该测定法证明不受59种药物的干扰，并且与采用电子捕获检测的气相色谱法的验证方法高度相关（斜率 = 0.90，截距 = 0.17，r = 0.98，n = 31）。作者得出结论，此处描述的方法非常适合白消安的治疗监测。
