Kitamura H, Matsuura E, Nagata A, Sakairi N, Tokura S, Nishi N
Division of Bioscience, Graduate school of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Int J Biol Macromol. 1997 Feb;20(1):75-7. doi: 10.1016/s0141-8130(97)01146-x.
Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected rather than to poly-L-lysine. These results shown an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
