用紫外线处理过的聚苯乙烯微量滴定板对双链DNA抗体进行酶联免疫吸附测定的重新评估。

Reevaluation of enzyme-linked immunosorbent assay with ultraviolet-treated polystyrene microtiter plates for measurement of antibodies to dsDNA.

作者信息

Kaburaki J, Ogasawara T, Hayakawa M, Kuwana M, Tojo T, Ikeda Y

机构信息

Department of Internal Medicine, Keio University School of Medicine.

出版信息

Nihon Rinsho Meneki Gakkai Kaishi. 1996 Apr;19(2):163-7. doi: 10.2177/jsci.19.163.

DOI:10.2177/jsci.19.163

PMID:8705694

Abstract

We reevaluated the efficacy of enzyme-linked immunosorbent assay (ELISA) with ultraviolet (UV)-treated polystyrene microtiter plates (UV-ELISA) for the detection of human serum anti-dsDNA antibodies. The subjects consisted of 38 patients with systemic lupus erythematosus (SLE). The titers of IgG serum anti-dsDNA antibodies by UV-ELISA were significantly (p < 0.01) higher in sera from 26 active SLE patients than in those from 12 inactive SLE patients. ELISA with poly-L-lysine coated microtiter plates (PLL-ELISA) revealed the similar results. However, the background ratio was only 1.0 +/- 0.9% by UV-ELISA, which was significantly (p < 0.01) lower than that by PLL-ELISA (16.8 +/- 10.8%). These results demonstrated the efficacy of ELISA with UV-treated polystyrene microtiter plates for the measurement of human serum anti-dsDNA antibodies in patients with SLE.

摘要

我们重新评估了用紫外线（UV）处理过的聚苯乙烯微量滴定板进行酶联免疫吸附测定（ELISA）（UV-ELISA）检测人血清抗双链DNA（dsDNA）抗体的效果。研究对象包括38例系统性红斑狼疮（SLE）患者。UV-ELISA检测的26例活动期SLE患者血清中IgG抗dsDNA抗体滴度显著（p<0.01）高于12例非活动期SLE患者血清中的滴度。用聚-L-赖氨酸包被微量滴定板的ELISA（PLL-ELISA）显示了相似的结果。然而，UV-ELISA的背景率仅为1.0±0.9%，显著（p<0.01）低于PLL-ELISA的背景率（16.8±10.8%）。这些结果证明了用紫外线处理过的聚苯乙烯微量滴定板进行ELISA在检测SLE患者人血清抗dsDNA抗体方面的有效性。