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公猪精浆精子黏附蛋白PSP-II糖型的分级分离与表征揭示了含有罕见的N-乙酰半乳糖胺的N-连接寡糖的存在。

Fractionation and characterization of boar seminal plasma spermadhesion PSP-II glycoforms reveal the presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides.

作者信息

Solis D, Calvete J J, Sanz L, Hettel C, Raida M, Diaz-Mauriño T, Töpfer-Petersen E

机构信息

Instituto de Quimíca-Física Rocasolano CSIC, Madrid, Spain.

出版信息

Glycoconj J. 1997 Feb;14(2):275-80. doi: 10.1023/a:1018506208616.

DOI:10.1023/a:1018506208616
PMID:9111145
Abstract

Lectin mapping, carbohydrate analysis and electrospray mass spectrometry of boar seminal plasma PSP-II glycoforms show that its single N-glycosylation site displays a repertoire of carbohydrate structures consisting of the basic pentasaccharide core Man alpha 1-6[Man alpha 1-3]Man beta1-4GlcNAc beta1-4GlcNAc with a fucosyl residue alpha1-6-linked to the innermost N-acetylglucosamine residue. Other glycoforms display fucosylated hybrid-type or monoantennary complex-type chains, some of which contain alpha2-6-linked sialic acid. N-acetylgalactosamine, possibly in Gal beta1-3GalNAc sequence, is present in most of the PSP-II glycoforms.

摘要

对公猪精浆PSP-II糖型进行凝集素图谱分析、碳水化合物分析和电喷雾质谱分析表明，其单一的N-糖基化位点呈现出一系列碳水化合物结构，这些结构由基本的五糖核心Manα1-6[Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAc组成，其中一个岩藻糖基残基以α1-6连接至最内层的N-乙酰葡糖胺残基。其他糖型呈现出岩藻糖基化的杂合型或单天线复合型链，其中一些含有α2-6连接的唾液酸。N-乙酰半乳糖胺可能以Galβ1-3GalNAc序列存在于大多数PSP-II糖型中。

相似文献

1
Fractionation and characterization of boar seminal plasma spermadhesion PSP-II glycoforms reveal the presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides.公猪精浆精子黏附蛋白PSP-II糖型的分级分离与表征揭示了含有罕见的N-乙酰半乳糖胺的N-连接寡糖的存在。
Glycoconj J. 1997 Feb;14(2):275-80. doi: 10.1023/a:1018506208616.
2
Structural characterization of the oligosaccharide chains of native and crystallized boar seminal plasma spermadhesin PSP-I and PSP-II glycoforms.天然和结晶的公猪精浆精子黏附素PSP-I和PSP-II糖型寡糖链的结构表征
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本文引用的文献

1
Crystallization and preliminary X-ray diffraction analysis of boar seminal plasma spermadhesin PSP-I/PSP-II, a heterodimer of two CUB domains.公猪精浆精子黏附素PSP-I/PSP-II（两个CUB结构域的异二聚体）的结晶及初步X射线衍射分析
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Carbohydrate-and heparin-binding proteins in mammalian fertilization.哺乳动物受精过程中的碳水化合物结合蛋白和肝素结合蛋白。
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Analysis of the structural organization and thermal stability of two spermadhesins. Calorimetric, circular dichroic and Fourier-transform infrared spectroscopic studies.
两种精子黏附素的结构组织与热稳定性分析。量热法、圆二色光谱法和傅里叶变换红外光谱研究。
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Characterization of two glycosylated boar spermadhesins.两种糖基化公猪精子黏附素的特性分析
Eur J Biochem. 1993 Dec 1;218(2):719-25. doi: 10.1111/j.1432-1033.1993.tb18426.x.
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7
Characterization of AWN-1 glycosylated isoforms helps define the zona pellucida and serine proteinase inhibitor-binding region on boar spermadhesins.AWN-1糖基化异构体的特性有助于确定公猪精子黏附素上的透明带和丝氨酸蛋白酶抑制剂结合区域。
FEBS Lett. 1993 Nov 8;334(1):37-40. doi: 10.1016/0014-5793(93)81675-p.
8
Quantitation of boar spermadhesins in accessory sex gland fluids and on the surface of epididymal, ejaculated and capacitated spermatozoa.公猪精子黏附素在附属性腺液以及附睾、射出和获能精子表面的定量分析。
Biochim Biophys Acta. 1994 May 25;1200(1):48-54. doi: 10.1016/0304-4165(94)90026-4.
9
Glycosylated boar spermadhesin AWN-1 isoforms. Biological origin, structural characterization by lectin mapping, localization of O-glycosylation sites, and effect of glycosylation on ligand binding.糖基化的公猪精子黏附素AWN-1亚型。生物学来源、通过凝集素图谱进行的结构表征、O-糖基化位点的定位以及糖基化对配体结合的影响。
Biol Chem Hoppe Seyler. 1994 Oct;375(10):667-73. doi: 10.1515/bchm3.1994.375.10.667.
10
Boar spermadhesin PSP-II: location of posttranslational modifications, heterodimer formation with PSP-I glycoforms and effect of dimerization on the ligand-binding capabilities of the subunits.
FEBS Lett. 1995 May 29;365(2-3):179-82. doi: 10.1016/0014-5793(95)00452-f.