Hoefkens P, Huijskes-Heins M I, de Jeu-Jaspars C M, van Noort W L, van Eijk H G
Department of Chemical Pathology, Erasmus University Rotterdam, The Netherlands.
Glycoconj J. 1997 Feb;14(2):289-95. doi: 10.1023/a:1018510309524.
Human bi-bi-antennary transferrin (Tf) was partially deglycosylated by subsequently incubating with one or more of the following exoglycosidases: neuraminidase, beta-galactosidase or N-Acetyl-beta-D-glucosaminidase. Aglyco-Tf obtained from serum of a patient suffering from the Carbohydrate Deficient Glycoprotein syndrome was isolated. Receptor binding and the Tf and iron uptake capacities of the fully glycosylated-, partially deglycosylated- and aglyco-Tf were compared using the human hepatoma cell line PLC/PRF/5. No difference in binding capacity between the iso-Tf fractions could be demonstrated, however, the Tf and iron uptake capacity of aglyco-Tf was clearly reduced compared with the other Tf fractions.
人双触角转铁蛋白（Tf）通过与以下一种或多种外切糖苷酶孵育进行部分去糖基化：神经氨酸酶、β-半乳糖苷酶或N-乙酰-β-D-氨基葡萄糖苷酶。从患有碳水化合物缺乏糖蛋白综合征患者的血清中分离出无糖基Tf。使用人肝癌细胞系PLC/PRF/5比较了完全糖基化、部分去糖基化和无糖基Tf的受体结合以及Tf和铁摄取能力。各同工型Tf组分之间的结合能力没有差异，然而，与其他Tf组分相比，无糖基Tf的Tf和铁摄取能力明显降低。
