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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

van Eijk H G, van Noort W L, Kroos M J, van der Heul C

J Clin Chem Clin Biochem. 1980 Sep;18(9):563-6. doi: 10.1515/cclm.1980.18.9.563.

Human transferrin was labelled with Fe(III) in nitrilo triacetate buffer at one of its two metal binding sites by variation of the pH. The A-site was preferentially labelled at pH 7.2, the B-site at pH 9.2. 2. The monoferric transferrin preparations were subjected to preparative isoelectric focussing, isolated from the granulated gels, and checked for homogeneity and purity on an analytical scale by isoelectric focussing. 3. The technique for obtaining stable monoferric transferrin (Fe)(A) and transferrin (Fe)(B) preparations is described in detail.

通过改变pH值，在氮川三乙酸缓冲液中，人转铁蛋白在其两个金属结合位点之一被Fe(III)标记。在pH 7.2时，A位点优先被标记；在pH 9.2时，B位点优先被标记。2. 将单铁转铁蛋白制剂进行制备性等电聚焦，从颗粒状凝胶中分离出来，并通过等电聚焦在分析规模上检查其均一性和纯度。3. 详细描述了获得稳定的单铁转铁蛋白(Fe)(A)和转铁蛋白(Fe)(B)制剂的技术。

van Eijk H G, van Noort W L, Kroos M J, van der Heul C

J Clin Chem Clin Biochem. 1980 Sep;18(9):563-6. doi: 10.1515/cclm.1980.18.9.563.

Human transferrin was labelled with Fe(III) in nitrilo triacetate buffer at one of its two metal binding sites by variation of the pH. The A-site was preferentially labelled at pH 7.2, the B-site at pH 9.2. 2. The monoferric transferrin preparations were subjected to preparative isoelectric focussing, isolated from the granulated gels, and checked for homogeneity and purity on an analytical scale by isoelectric focussing. 3. The technique for obtaining stable monoferric transferrin (Fe)(A) and transferrin (Fe)(B) preparations is described in detail.

通过改变pH值，在氮川三乙酸缓冲液中，人转铁蛋白在其两个金属结合位点之一被Fe(III)标记。在pH 7.2时，A位点优先被标记；在pH 9.2时，B位点优先被标记。2. 将单铁转铁蛋白制剂进行制备性等电聚焦，从颗粒状凝胶中分离出来，并通过等电聚焦在分析规模上检查其均一性和纯度。3. 详细描述了获得稳定的单铁转铁蛋白(Fe)(A)和转铁蛋白(Fe)(B)制剂的技术。