Sincock P M, Mayrhofer G, Ashman L K
Department of Medicine, The University of Adelaide, Australia.
J Histochem Cytochem. 1997 Apr;45(4):515-25. doi: 10.1177/002215549704500404.
It has recently been shown that several members of the tetraspan superfamily, including CD9 and CD63, associate with each other and with beta1 integrins. In this study, we examined the distribution of a recently identified tetraspan, PETA-3 (CD151), and of CD9, CD63, alpha5beta1, and the integrin beta1 chain in normal human tissues by the indirect immunoperoxidase and alkaline phosphatase-anti-alkaline phosphatase techniques. PETA-3 showed a broad distribution and was expressed by endothelium, epithelium, Schwann cells, and dendritic cells and by skeletal, smooth, and cardiac muscle. Expression in skin was mostly restricted to the basal cells of the epidermis and was downregulated on differentiation. In the small intestine, PETA-3 was expressed by crypt and villous enterocytes with a mostly basolateral distribution, but was not detectable on the brush border. CD9 was expressed on the plasma membrane of enterocytes in crypts and at the bases of the villi whereas CD63 demonstrated a unique granular appearance concentrated in the apical cytoplasm below the brush border. The findings of this study show co-localization of PETA-3 with CD9, CD63, alpha5beta1, and beta1 in particular tissues, demonstrating that tetraspan/integrin complexes may occur. However, the lack of co-localization of these antigens in other tissues also implies distinct roles for these molecules.
最近研究表明，四跨膜超家族的几个成员，包括CD9和CD63，彼此之间以及与β1整合素相互关联。在本研究中，我们采用间接免疫过氧化物酶法和碱性磷酸酶-抗碱性磷酸酶技术，检测了一种最近鉴定出的四跨膜蛋白PETA-3（CD151）以及CD9、CD63、α5β1和整合素β1链在正常人体组织中的分布情况。PETA-3分布广泛，在内皮细胞、上皮细胞、雪旺细胞、树突状细胞以及骨骼肌、平滑肌和心肌中均有表达。在皮肤中的表达主要局限于表皮的基底细胞，且在分化过程中表达下调。在小肠中，PETA-3由隐窝和绒毛肠上皮细胞表达，大多分布于基底外侧，但在刷状缘未检测到。CD9在隐窝和绒毛底部的肠上皮细胞质膜上表达，而CD63呈现出独特的颗粒状外观，集中在刷状缘下方的顶端细胞质中。本研究结果表明，PETA-3与CD9、CD63、α5β1和β1在特定组织中共定位，表明可能存在四跨膜蛋白/整合素复合物。然而，这些抗原在其他组织中缺乏共定位也意味着这些分子具有不同的作用。
