KleinJan A, Godthelp T, van Toornenenbergen A W, Fokkens W J
Department of Otorhinolaryngology, University Hospital Dijkzigt, Rotterdam, The Netherlands.
J Allergy Clin Immunol. 1997 Apr;99(4):515-21. doi: 10.1016/s0091-6749(97)70079-4.
Until now, it has not been possible to identify specific IgE locally in the airway mucosa. In this study we investigated the possibility of determining specific allergen binding locally in the nasal mucosa.
Nasal mucosal biopsy specimens were taken from 11 patients with symptoms of an isolated grass pollen allergy, 10 patients with symptoms of perennial allergic rhinitis in response to house dust mite allergen, and 10 nonallergic control subjects. Sections of these biopsy specimens were stained by using commercially available biotinylated allergens (AlaSTAT, Diagnostic Products Corp.).
Staining with biotinylated grass pollen (GP1) demonstrated positive cells only in patients with grass pollen allergy. Biotinylated Dermatophagoides pteronyssinus (D1) only stained cells in patients with perennial allergy. Specific binding of allergen to cells of patients with allergy and the blocking experiments proved the method to be highly specific. Allergen-positive cells stained double with IgE, the high-affinity receptor for IgE (Fc epsilon RI), CD1, HLA-DR, tryptase, and chymase. Most allergen-positive cells proved to be mast cells.
This immunohistochemical study shows the presence of specific IgE against grass pollen and house dust mite allergens locally on cells in the airway mucosa.
迄今为止，尚无法在气道黏膜局部鉴定特异性IgE。在本研究中，我们调查了在鼻黏膜局部测定特异性变应原结合的可能性。
从11例单纯草花粉过敏症状患者、10例对屋尘螨变应原产生常年性变应性鼻炎症状的患者以及10例非变应性对照受试者中获取鼻黏膜活检标本。使用市售生物素化变应原（AlaSTAT，诊断产品公司）对这些活检标本切片进行染色。
用生物素化草花粉（GP1）染色仅在草花粉过敏患者中显示出阳性细胞。生物素化粉尘螨（D1）仅对常年性过敏患者的细胞进行染色。变应原与过敏患者细胞的特异性结合及阻断实验证明该方法具有高度特异性。变应原阳性细胞与IgE、IgE高亲和力受体（FcεRI）、CD1、HLA - DR、类胰蛋白酶和糜酶呈双重染色。大多数变应原阳性细胞被证明是肥大细胞。
这项免疫组织化学研究表明，气道黏膜中的细胞局部存在针对草花粉和屋尘螨变应原的特异性IgE。
