Production of beta-mannanase by B. subtilis from agro-industrial by-products: screening and optimization. Mannanase from wastes.

We have demonstrated that a mixture of wheat bran (35 g l-1), as a main substrate, and palm seed powder (10 g l-1), as a co-substrate, is appropriate for beta-mannanase production by Bacillus subtilis. A 2n factorial experimental design was employed as a primary step for medium optimization. The enzyme activity titters obtained at the optimized growth condition were equivalent to about 319% of the beta-mannanase activity and 114% of the specific activity levels reached by a galactomannan-based culture.