Weekers P H, De Jonckheere J F
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
Antonie Van Leeuwenhoek. 1997 Mar;71(3):231-7. doi: 10.1023/a:1000185110677.
Axenically and monoxenically grown Acanthamoeba castellanii, Acanthamoeba polyphaga and different isolates of Hartmannella vermiformis strains were examined by polyacrylamide isoelectric focusing in the pH range 3-10. Isoenzyme patterns of acid phosphatase (AP), propionyl esterase (PE), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glucose phosphate isomerase (GPI) and phosphoglucomutase (PGM) were compared. Zymograms were used to reveal differences in typical isoenzyme patterns between axenically and monoxenically grown amoebae and to compare axenically grown A. castellanii, A. polyphaga and H. vermiformis. Comparison of zymograms for AP, PE and MDH between axenically grown Acanthamoeba and Hartmannella strains revealed different isoenzyme patterns. Acanthamoeba showed strong bands for ADH and extremely weak bands for GPI and PGM, while Hartmannella lacked ADH but possessed bands for GPI and PGM. Comparison of zymograms from axenically and monoxenically grown amoebae revealed a lower intensity and even lack of typical isoenzyme bands in lysates from monoxenic cultures. The observed changes in typical isoenzyme patterns induced by the bacterial substrate can influence the correct isoenzymatic typing of different strains in clinical and phylogenetic studies.
采用聚丙烯酰胺等电聚焦法，在pH值3 - 10范围内，对无菌培养和单菌培养的卡氏棘阿米巴、多食棘阿米巴以及不同分离株的蠕虫状哈特曼原虫菌株进行了检测。比较了酸性磷酸酶（AP）、丙酰酯酶（PE）、苹果酸脱氢酶（MDH）、乙醇脱氢酶（ADH）、葡萄糖磷酸异构酶（GPI）和磷酸葡萄糖变位酶（PGM）的同工酶谱。酶谱用于揭示无菌培养和单菌培养的变形虫之间典型同工酶谱的差异，并比较无菌培养的卡氏棘阿米巴、多食棘阿米巴和蠕虫状哈特曼原虫。无菌培养的棘阿米巴和哈特曼原虫菌株之间AP、PE和MDH酶谱的比较显示出不同的同工酶谱。棘阿米巴显示出ADH的强条带以及GPI和PGM的极弱条带，而哈特曼原虫缺乏ADH，但具有GPI和PGM的条带。无菌培养和单菌培养的变形虫酶谱的比较显示，单菌培养物裂解物中典型同工酶条带的强度较低，甚至缺失。观察到的由细菌底物诱导的典型同工酶谱变化可能会影响临床和系统发育研究中不同菌株的正确同工酶分型。
