Moss D M, Brandt F H, Mathews H M, Visvesvara G S
U.S. Department of Health and Human Services, Centers for Disease Control, Atlanta, Georgia 30333.
J Protozool. 1988 Feb;35(1):26-31. doi: 10.1111/j.1550-7408.1988.tb04070.x.
High-resolution polyacrylamide gradient gel electrophoresis (PGGE) was used to separate isoenzymes of 12 Naegleria strains: one N. australiensis, two N. lovaniensis, one N. jadini, two N. gruberi isolated from environmental samples, and six N. fowleri strains isolated from patients with primary amoebic meningoencephalitis. Of the eight enzymes studied, seven showed zymograms with interspecific variation that identified all the species tested. Although the six N. fowleri strains were biochemically the most homogeneous, they showed intraspecific isoenzyme variation that allowed them to be grouped into four zymodemes. The PGGE technique, which separates isoenzymes by their molecular shape, is both sensitive and economical. It offers an addition or an attractive alternative to isoelectric focusing which has commonly been used to aid species identification of Naegleria by separating isoenzymes by their isoelectric point.
采用高分辨率聚丙烯酰胺梯度凝胶电泳（PGGE）对12株耐格里属菌株的同工酶进行分离：1株澳大利亚耐格里阿米巴、2株洛文耐格里阿米巴、1株贾丁耐格里阿米巴、从环境样本中分离出的2株格氏耐格里阿米巴，以及从原发性阿米巴脑膜脑炎患者中分离出的6株福氏耐格里阿米巴。在所研究的8种酶中，有7种显示出种间变异的酶谱，可识别所有测试的物种。虽然6株福氏耐格里阿米巴菌株在生化方面最为同质，但它们显示出种内同工酶变异，可将它们分为4个酶型。PGGE技术通过分子形状分离同工酶，既灵敏又经济。它为等电聚焦提供了一种补充或有吸引力的替代方法，等电聚焦通常用于通过按等电点分离同工酶来辅助耐格里属物种的鉴定。
