Takeuchi K, Takechi S, Ohoka H, Yokoyama M, Iwata H, Takeuchi M, Matsuda S
Department of Urology, Ehime University School of Medicine, Shitsukawa, Shigenobu-cho, Japan.
Transplantation. 1997 Apr 15;63(7):922-6. doi: 10.1097/00007890-199704150-00002.
Patients who require cystectomy are usually treated with an ileal conduit or intestinal neobladder for urinary control. In some of them, however, the bowel segment cannot be used because of previous abdominal surgery or radiation treatment. Bladder transplantation from cadavers may be beneficial to these patients, if possible. To obtain basic knowledge about bladder transplantation, we developed an animal model of whole bladder transplantation in rats. Male Lewis rats weighing 270-320 g were used as both donors and recipients. Of the 23 recipients, 12 (52.2%) survived 7 days or longer after surgery. At 1 week after transplantation, the bladder showed loss of transitional epithelium and remarkable cellular infiltration. In the bladder at 5 weeks after transplantation, the transitional epithelium regenerated markedly and submucosal cellular infiltration was much improved. Regeneration of some smooth muscle cells was also noted. At 6 months after transplantation, the nerve fibers were recognized in the bladder and the volume of the transplanted bladder was well preserved (1.0-1.3 ml). This article describes an animal model of whole bladder transplantation in the rat which we produced and the results of our study. Because a large number of pure-bred animals can easily be used, we believe our rat model is very useful for basic studies of bladder transplantation.
需要进行膀胱切除术的患者通常采用回肠导管或肠道新膀胱来控制排尿。然而，在其中一些患者中，由于先前的腹部手术或放射治疗，肠道段无法使用。如果可能的话，尸体膀胱移植可能对这些患者有益。为了获取有关膀胱移植的基础知识，我们建立了大鼠全膀胱移植的动物模型。体重270 - 320克的雄性Lewis大鼠用作供体和受体。23只受体中，12只（52.2%）在手术后存活7天或更长时间。移植后1周，膀胱出现移行上皮丧失和明显的细胞浸润。移植后5周的膀胱中，移行上皮明显再生，黏膜下细胞浸润明显改善。还观察到一些平滑肌细胞的再生。移植后6个月，膀胱中可识别出神经纤维，移植膀胱的体积保存良好（1.0 - 1.3毫升）。本文描述了我们制作的大鼠全膀胱移植动物模型及其研究结果。由于可以轻松使用大量纯种动物，我们认为我们的大鼠模型对膀胱移植的基础研究非常有用。
