ADP核糖基化因子1与外被体亚基β的直接且依赖鸟苷三磷酸的相互作用。
Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta.
作者信息
Zhao L, Helms J B, Brügger B, Harter C, Martoglio B, Graf R, Brunner J, Wieland F T
机构信息
Biochemie-Zentrum Heidelberg, Universität Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
出版信息
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4418-23. doi: 10.1073/pnas.94.9.4418.
Abstract
A site-directed photocrosslink approach was used to elucidate components that interact directly with ADP- ribosylation factor (ARF)-GTP during coat assembly. Two ARF mutants were generated that contain a photolabile amino acid at positions distant to each other within the ARF molecule. Here we show that one of the two positions specifically interacts with coatomer subunit beta both on Golgi membranes and in isolated coat protein complex type I (COPI)-coated vesicles. Thus, a direct and GTP-dependent interaction of coatomer via beta-coat protein complex (COP) with ARF is involved in the coating of COPI-coated vesicles. These data implicate a bivalent interaction of the complex with the donor membrane during vesicle formation.
摘要
采用定点光交联方法来阐明在衣被组装过程中与ADP-核糖基化因子（ARF）-GTP直接相互作用的成分。构建了两个ARF突变体，它们在ARF分子内彼此相距较远的位置含有一个光不稳定氨基酸。我们在此表明，这两个位置之一在高尔基体膜上以及在分离的I型衣被蛋白复合物（COPI）包被的囊泡中均与衣被蛋白复合物β亚基特异性相互作用。因此，衣被蛋白通过β-衣被蛋白复合物（COP）与ARF的直接且依赖GTP的相互作用参与了COPI包被囊泡的衣被形成。这些数据表明该复合物在囊泡形成过程中与供体膜存在二价相互作用。
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