通过聚合酶链反应检测基因多样的人类免疫缺陷病毒1型M组和O组毒株
Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.
作者信息
Respess R A, Butcher A, Wang H, Chaowanachan T, Young N, Shaffer N, Mastro T D, Biryahwaho B, Downing R, Tanuri A, Schechter M, Pascu R, Zekeng L, Kaptué L, Gürtler L, Eberle J, Ellenberger D, Fridlund C, Rayfield M, Kwok S
机构信息
Roche Molecular Systems, Inc., Alameda, California 94501, USA.
出版信息
J Clin Microbiol. 1997 May;35(5):1284-6. doi: 10.1128/jcm.35.5.1284-1286.1997.
Abstract
A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.
摘要
采用罗氏AMPLICOR HIV-1检测法、该检测法的改良版以及一种新的引物对/探针系统，通过聚合酶链式反应（PCR）对来自美国和欧洲以外地区的136株基因多样的M组和5株O组成年HIV分离株进行了评估。使用AMPLICOR HIV-1检测法检测其中一些分离株的效率较低；然而，通过减少样本输入量和降低退火温度，该检测方法有了显著改进。新的引物对/探针组检测出了141株分离株中的140株，包括使用两种AMPLICOR HIV-1检测法均未检测出的5株O组分离株。
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