Loussert-Ajaka I, Ly T D, Chaix M L, Ingrand D, Saragosti S, Couroucé A M, Brun-Vézinet F, Simon F
Virology Department, Bichat-Claude Bernard Hospital, Paris, France.
Lancet. 1994 Jun 4;343(8910):1393-4. doi: 10.1016/s0140-6736(94)92524-0.
Nine patients with atypical HIV-1 western blot profiles were diagnosed as having HIV-1 subtype O infection. All the patients were living in France; eight originated from Cameroon and one from France. Lymphocyte DNA amplification by the polymerase chain reaction was only positive when HIV-1 subtype O specific primers were used. Preliminary sequence analysis of amplified products and serological reactivity against a specific subtype O synthetic env peptide confirmed HIV-1 subtype O infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially those based on env peptides or on the sandwich format, can be negative in HIV-1 subtype O infection.
9例具有非典型HIV-1免疫印迹图谱的患者被诊断为感染了HIV-1 O亚型。所有患者均居住在法国；其中8例来自喀麦隆，1例来自法国。仅在使用HIV-1 O亚型特异性引物时，通过聚合酶链反应进行的淋巴细胞DNA扩增才呈阳性。对扩增产物的初步序列分析以及针对特定O亚型合成env肽的血清学反应性证实了HIV-1 O亚型感染。HIV-1/HIV-2酶联免疫吸附试验，尤其是基于env肽或夹心形式的试验，在HIV-1 O亚型感染中可能为阴性。
