• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Hybridization properties of sequences adjacent to triphosphorylated 5'-ends of nuclear pre-mRNA from mouse Ehrlich carcinoma.来自小鼠艾氏癌细胞核前体mRNA三磷酸化5'-末端相邻序列的杂交特性
Nucleic Acids Res. 1979 Aug 10;6(11):3625-40. doi: 10.1093/nar/6.11.3625.
2
[Structure of nuclear pre-mRNA. XIII. Hybridization properties of triphosphorylated 5'-end fragments].[核内前体信使核糖核酸的结构。十三。三磷酸化5′末端片段的杂交特性]
Mol Biol (Mosk). 1980;14(1):212-22.
3
[Structure of nuclear pre-mRNA. XI. Triphosphorylated and blocked 5'-ends in the pre-mRNA].[核前体信使核糖核酸的结构。XI。前体信使核糖核酸中的三磷酸化和封闭的5'末端]
Mol Biol (Mosk). 1979 Jan-Feb;13(1):60-72.
4
The properties of oligonucleotide fragments containing the triphosphorylated 5'-termini of nuclear pre-mRNA from Ehrlich ascites carcinoma cells.来自艾氏腹水癌细胞的核内前体信使核糖核酸中含有三磷酸化5′末端的寡核苷酸片段的性质。
Mol Biol Rep. 1976 Apr;2(5):369-77. doi: 10.1007/BF00366258.
5
Kinetic estimation of the base sequence complexity of poly(A)-containing mRNA in Ehrlich-ascites-tumor cells and its abundance in nuclear RNA.艾氏腹水瘤细胞中含聚腺苷酸信使核糖核酸碱基序列复杂性的动力学估计及其在核核糖核酸中的丰度
Eur J Biochem. 1979 Feb 15;94(1):51-7. doi: 10.1111/j.1432-1033.1979.tb12870.x.
6
On the nature of 5' termini in nuclear pre-mRNA of Ehrlich carcinoma cells.艾氏癌细胞核内前体信使核糖核酸5'末端的性质
Cell. 1976 Oct;9(2):323-32. doi: 10.1016/0092-8674(76)90122-7.
7
Sequence complexity and abundance classes of nuclear and polysomal polyadenylated RNA in Neurospora crassa.粗糙脉孢菌中核和多核糖体多聚腺苷酸化RNA的序列复杂性和丰度类别
Biochim Biophys Acta. 1980 Mar 28;607(1):122-35. doi: 10.1016/0005-2787(80)90226-9.
8
[Mapping of 2 cloned DNA fragments containing repetitive mouse genome regions, actively transcribing into pre-mRNA].[对两个含有小鼠基因组重复区域且正转录为前体信使核糖核酸的克隆DNA片段的定位]
Genetika. 1982 May;18(5):713-23.
9
Nucleotide sequence of small polyadenylated B2 RNA.小聚腺苷酸化B2 RNA的核苷酸序列。
Nucleic Acids Res. 1985 Sep 25;13(18):6423-37. doi: 10.1093/nar/13.18.6423.
10
Sequences hybridizing to mRNA, oligo(dT) and dsRNA from pre-mRNA are contiguous in the cloned mouse DNA fragments.与mRNA、寡聚（dT）以及来自前体mRNA的双链RNA杂交的序列，在克隆的小鼠DNA片段中是连续的。
Nucleic Acids Res. 1980 Feb 11;8(3):425-40. doi: 10.1093/nar/8.3.425.

本文引用的文献

1
Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells.在海拉细胞中证实存在不稳定RNA和核糖体RNA前体。
Proc Natl Acad Sci U S A. 1963 Feb 15;49(2):240-8. doi: 10.1073/pnas.49.2.240.
2
[Messenger and ribosomal ribonucleic acid in the chromosomal-nucleolar apparatus, methods of isolation and nuceotide structure].[染色体核仁装置中的信使核糖核酸和核糖体核糖核酸、分离方法及核苷酸结构]
Biokhimiia. 1962 Sep-Oct;27:949-57.
3
Isolation of a nuclear RNA fraction resembling DNA in its base composition.分离出一种碱基组成类似于DNA的核RNA组分。
Proc Natl Acad Sci U S A. 1962 Mar 15;48(3):471-7. doi: 10.1073/pnas.48.3.471.
4
Polyphosphate groups at the 5'-ends of nuclear dRNA fractions.核内不均一RNA组分5′端的多磷酸基团。
FEBS Lett. 1970 Jun 8;8(4):186-188. doi: 10.1016/0014-5793(70)80259-9.
5
Messenger RNA in avian erythroblasts at the transcriptional and translational levels and the problem of regulation in animal cells.禽类成红细胞中转录和翻译水平的信使核糖核酸以及动物细胞中的调控问题。
J Cell Physiol. 1968 Oct;72(2):Suppl 1:181+. doi: 10.1002/jcp.1040720413.
6
On the structural organization of operon and the regulation of RNA synthesis in animal cells.关于操纵子的结构组织及动物细胞中RNA合成的调控
J Theor Biol. 1969 Dec;25(3):473-90. doi: 10.1016/s0022-5193(69)80034-2.
7
Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.在腺病毒复制过程中，向病毒特异性RNA添加聚腺苷酸序列。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
8
On the structure of transcriptional unit in mammalian cells.关于哺乳动物细胞中转录单位的结构。
Biochim Biophys Acta. 1972 Jan 31;259(2):259-83. doi: 10.1016/0005-2787(72)90066-4.
9
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。
Science. 1968 Aug 9;161(3841):529-40. doi: 10.1126/science.161.3841.529.
10
Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.哺乳动物蛋白质合成的起始：核糖体和起始因子质量对体外系统效率的重要性。
J Mol Biol. 1973 Feb 19;73(3):329-49. doi: 10.1016/0022-2836(73)90346-x.

来自小鼠艾氏癌细胞核前体mRNA三磷酸化5'-末端相邻序列的杂交特性

Hybridization properties of sequences adjacent to triphosphorylated 5'-ends of nuclear pre-mRNA from mouse Ehrlich carcinoma.

作者信息

Peunova N I, Samarina O P, Georgiev G P

出版信息

Nucleic Acids Res. 1979 Aug 10;6(11):3625-40. doi: 10.1093/nar/6.11.3625.

DOI:10.1093/nar/6.11.3625
PMID:91157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327961/
Abstract

Triphosphorylated 5'-end fragments about 100 nucleotides long were prepared from purified nuclear pre-mRNA using a modified hydroxyapatite method /1/. These fragments as well as fragments of total pre-mRNA of the same size were polyadenylated in vitro by ATP:RNA adenyltransferase and used as templates for the synthesis of [32P] cDNA by reverse transcriptase in the presence of an oligo(dT) primer. The use of cDNA transcribed from the triphosphorylated 5'-end fragments of pre-mRNA (5'-cDNA) and from the total pre-mRNA fragments allows one to calculate the complexity of the 5'-end fraction pre-mRNA and to detect these sequences in polysomal mRNA. Sequences adjacent to 5'-phosphorylated ends of pre-mRNA represent a specific class of sequences with a complexity of about 200 kb. It was also found that about 25% of total pre-mRNA and about a half of sequences adjacent to triphosphorylated 5'-ends are present in polysomal mRNA. A high homology between triphosphorylated 5'-end fragments of pre-mRNA and mRNA sequences may be explained in terms of splicing. Less than 30% of 5'-cDNA hybridized to moderately repetitive DNA while most of them are represented by unique DNA sequences. About 15% of 5'-cDNA contained oligo(dA) sequences originated from oligo(U) in pre-mRNA from which it was transcribed.

摘要

使用改良的羟基磷灰石方法从纯化的核前体mRNA制备了长度约为100个核苷酸的三磷酸化5'-末端片段/1/。这些片段以及相同大小的总前体mRNA片段在体外通过ATP:RNA腺苷酸转移酶进行聚腺苷酸化，并在寡聚(dT)引物存在下用作逆转录酶合成[32P]cDNA的模板。使用从mRNA前体的三磷酸化5'-末端片段（5'-cDNA）和总前体mRNA片段转录的cDNA，可以计算前体mRNA 5'-末端部分的复杂度，并在多核糖体mRNA中检测这些序列。与前体mRNA 5'-磷酸化末端相邻的序列代表一类特定的序列，其复杂度约为200 kb。还发现，总前体mRNA的约25%和约一半与三磷酸化5'-末端相邻的序列存在于多核糖体mRNA中。前体mRNA的三磷酸化5'-末端片段与mRNA序列之间的高度同源性可以用剪接来解释。不到30%的5'-cDNA与中度重复DNA杂交，而它们中的大多数由独特的DNA序列代表。约15%的5'-cDNA含有源自其转录的前体mRNA中的寡聚(U)的寡聚(dA)序列。