在腺病毒复制过程中，向病毒特异性RNA添加聚腺苷酸序列。
Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.
作者信息
Philipson L, Wall R, Glickman G, Darnell J E
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
Abstract
Adenovirus-specific nuclear and polysomal RNA, both early and late in the infectious cycle, contain a covalently linked region of polyadenylic acid 150-250 nucleotides long. A large proportion of the adenovirus-specific messenger RNA contains poly(A). As revealed by hybridization experiments, the poly(A) is not transcribed from adenovirus DNA. Furthermore, an adenosine analogue, cordycepin, blocks the synthesis of poly(A) and also inhibits the accumulation of adenovirus messenger RNA on polysomes. Addition of poly(A) to viral RNA may involve a host-controlled mechanism that regulates the processing and transport of messenger RNA.
摘要
在感染周期的早期和晚期，腺病毒特异性的核RNA和多聚核糖体RNA都含有一个长度为150 - 250个核苷酸的共价连接的聚腺苷酸区域。大部分腺病毒特异性信使RNA都含有聚腺苷酸。杂交实验表明，聚腺苷酸并非由腺病毒DNA转录而来。此外，腺苷类似物虫草素可阻断聚腺苷酸的合成，还能抑制腺病毒信使RNA在多聚核糖体上的积累。向病毒RNA添加聚腺苷酸可能涉及一种宿主控制的机制，该机制调节信使RNA的加工和转运。
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