产前与乳蛋白的过敏原接触。

Prenatal allergen contact with milk proteins.

作者信息

Szépfalusi Z, Nentwich I, Gerstmayr M, Jost E, Todoran L, Gratzl R, Herkner K, Urbanek R

机构信息

Department of Pediatrics, University of Vienna, Austria.

出版信息

Clin Exp Allergy. 1997 Jan;27(1):28-35.

PMID:9117877

Abstract

BACKGROUND

Cellular proliferation to various allergens (Dermatophagoides pteronyssinus, beta-lactoglobulin, bovine serum albumin, ovalbumin) has been found in cord blood cells. Whether this reflects a sensitization during foetal life is uncertain.

OBJECTIVE

We studied the cellular reactivity and cytokine production of cord blood cells in response to cow's milk proteins in a randomly selected group of newborns. The delineation of possible in utero allergen contact was attempted.

METHODS

Cord blood mononuclear cells from 39 neonates were incubated with cow's milk proteins (alpha-lactalbumin, beta-lactoglobulin, casein, alpha-casein, beta-casein, kappa-casein, bovine serum albumin) for 7 days, and proliferation was assessed by incorporation of [3H]thymidine. Cord blood cell-derived interferon-gamma (IFN gamma) and interleukin-4 (IL-4) secretion was evaluated in response to allergen or phytohaemagglutinin (PHA) stimulation.

RESULTS

A pronounced proliferation of cells stimulated with alpha-lactalbumin (ALA: mean stimulation index 8.0, 95% confidence interval 5.2-10.8), beta-lactoglobulin (BLG: mean stimulation index 5.9, 95% confidence interval 3.2-8.6) and alpha-casein (2.6, 95% confidence interval 2.9-9.1), as opposed to unstimulated cells in medium, was found. No correlation was found between cellular proliferation to milk proteins and parental atopy, maternal total IgE or cord blood IgE. IFN gamma production (but not IL-4) was inducible by PHA (range 429-1810 pg/ml), but only in one individual upon stimulation with BLG. Preferentially, reduced IFN gamma levels were found in individuals with positive parental allergic history.

CONCLUSION

The recognition of allergen by cord blood cells indicates that allergen priming must occur prenatally. The relevance for subsequent sensitization is unclear.

摘要

背景

已发现脐血细胞对多种变应原（屋尘螨、β-乳球蛋白、牛血清白蛋白、卵清蛋白）有细胞增殖反应。这是否反映胎儿期致敏尚不确定。

目的

我们研究了一组随机选择的新生儿脐血细胞对牛奶蛋白的细胞反应性和细胞因子产生情况。试图明确可能的宫内变应原接触情况。

方法

将39例新生儿的脐血单个核细胞与牛奶蛋白（α-乳白蛋白、β-乳球蛋白、酪蛋白、α-酪蛋白、β-酪蛋白、κ-酪蛋白、牛血清白蛋白）孵育7天，通过掺入[3H]胸腺嘧啶核苷评估增殖情况。评估脐血细胞在变应原或植物血凝素（PHA）刺激下产生干扰素-γ（IFNγ）和白细胞介素-4（IL-4）的分泌情况。

结果

发现用α-乳白蛋白（ALA：平均刺激指数8.0，95%置信区间5.2 - 10.8）、β-乳球蛋白（BLG：平均刺激指数5.9，95%置信区间3.2 - 8.6）和α-酪蛋白（2.6，95%置信区间2.9 - 9.1）刺激的细胞与培养基中未刺激的细胞相比有明显增殖。未发现细胞对牛奶蛋白的增殖与父母特应性、母亲总IgE或脐血IgE之间存在相关性。PHA可诱导IFNγ产生（范围429 - 1810 pg/ml），但仅在1例个体中BLG刺激后可诱导产生。优先在有阳性父母过敏史的个体中发现IFNγ水平降低。