Wong M L, Xin W W, Duman R S
Clinical Neuroendocrinology Branch, NIMH/NIH, Bethesda, MD 20892-1284, USA.
Mol Psychiatry. 1996 May;1(2):133-40.
We report here the isolation and localization of RLCR1, a rat G-protein-coupled receptor, which is the homologue (94% amino acid identity) of the bovine receptor (LCR1) isolated from a locus coeruleus cDNA library. The deduced amino acid sequence of the RLCR1 encodes a protein of 347 amino acids and yields a hydropathicity profile characteristic of receptors with seven putative transmembrane regions. The structure of the receptor protein encoded by RLCR1 is most similar to that of the Burkitt's lymphoma receptor 2 (43% identical) and C-C chemokine receptor type 1 (38%). The mRNA corresponding to the cDNA was detected in various rat tissues including spleen, liver, heart, and brain. In situ hybridization analysis shows that RLCR1 is distributed both in neurons and glia in the adult rat brain. Levels of RLCR1 mRNA are highest in the ependymal layer as previously reported. In addition, we found that RLCR1 is also expressed at lower levels in cerebral cortex, hippocampus, cerebellum, and brainstem. The finding that LCR1 is expressed in the adult indicates that this receptor influences neuronal function at times after maturation of the brain.
我们在此报告大鼠G蛋白偶联受体RLCR1的分离与定位，它是从蓝斑cDNA文库中分离出的牛受体（LCR1）的同源物（氨基酸序列一致性为94%）。RLCR1推导的氨基酸序列编码一个由347个氨基酸组成的蛋白质，并产生具有七个推定跨膜区域的受体特征性亲水性图谱。RLCR1编码的受体蛋白结构与伯基特淋巴瘤受体2最为相似（一致性为43%），与C-C趋化因子受体1型相似（一致性为38%）。在包括脾脏、肝脏、心脏和大脑在内的多种大鼠组织中检测到了与该cDNA对应的mRNA。原位杂交分析表明，RLCR1在成年大鼠大脑的神经元和神经胶质细胞中均有分布。如先前报道，RLCR1 mRNA水平在室管膜层最高。此外，我们发现RLCR1在大脑皮层、海马体、小脑和脑干中也有较低水平的表达。LCR1在成年个体中表达这一发现表明，该受体在大脑成熟后的某些时候会影响神经元功能。
