Peekhaus N, Tong S, Reizer J, Saier M H, Murray E, Conway T
Department of Microbiology, Ohio State University, Columbus, USA.
FEMS Microbiol Lett. 1997 Feb 15;147(2):233-8. doi: 10.1111/j.1574-6968.1997.tb10247.x.
The nucleotide sequences of seven Escherichia coli genes that encode members of the gluconate permease (GntP) family have recently become available. These genes include gntP, gntU, gntW, ORf449, dsdX, and ORFo454. The deduced amino acid sequences of all seven E. coli genes are homologous to the gntP gene products from Bacillus subtilis and B. licheniformis as well as two additional gene products from Haemophilus influenzae. These 11 proteins are not demonstrably homologous to members of the major facilitator superfamily or other recognized permease families. Four of the E. coli gluconate transporter genes have been cloned and shown to encode gluconate transporters with apparent affinities ranging from 6 to 212 microM. These studies serve to characterize a novel family of bacterial permeases.
最近已获得编码葡萄糖酸盐通透酶（GntP）家族成员的7个大肠杆菌基因的核苷酸序列。这些基因包括gntP、gntU、gntW、ORf449、dsdX和ORFo454。所有7个大肠杆菌基因推导的氨基酸序列与枯草芽孢杆菌和地衣芽孢杆菌的gntP基因产物以及流感嗜血杆菌的另外两个基因产物同源。这11种蛋白质与主要易化子超家族或其他公认的通透酶家族成员没有明显的同源性。4个大肠杆菌葡萄糖酸盐转运基因已被克隆，并显示编码的葡萄糖酸盐转运蛋白的表观亲和力范围为6至212微摩尔。这些研究有助于表征一个新的细菌通透酶家族。
