Wang Yiqiang, Niu Jingyi
State Key Lab Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, 5 Yan'erdao Road, Qingdao 266071, Shandong, China.
Immunol Lett. 2008 Aug 15;119(1-2):57-61. doi: 10.1016/j.imlet.2008.04.003. Epub 2008 May 14.
Recent studies proposed that besides their role in thrombosis, platelets are involved in modulation of immune response of organisms to foreign bodies through platelet-leukocyte cross-talks at different levels. In the present study, we compared the response of T and B lymphocytes to mitogens in the presence or absence of platelets in cell cultures. Proliferation of T cells in response to lower concentrations of anti-CD3 or ConA stimulation as well as IL2 production of ConA-induced T blasts were inhibited by platelets. Similarly, proliferation and IL6 production of B blasts stimulated with low dose lipopolysaccharide (LPS) or CpG oligodeoxynucleotide 1826 were also dramatically inhibited by platelets. Over-expression of early activation marker CD69 induced by mitogens was blocked by platelets in both T blasts and B blasts. Platelets in culture also blocked production of IgM and IgE in B cells that were induced by anti-CD40/IL4 or LPS/IL4 treatments. These observations provided new evidence for the theory that platelets play more complicated roles in immune compartments. More efforts should be made to address the issue whether such platelet-lymphocyte interactions have any physiological significance in human and animals.
最近的研究表明，血小板除了在血栓形成中发挥作用外，还通过不同水平的血小板 - 白细胞相互作用参与调节机体对外来物体的免疫反应。在本研究中，我们比较了细胞培养中存在或不存在血小板时T淋巴细胞和B淋巴细胞对有丝分裂原的反应。血小板抑制了T细胞对较低浓度抗CD3或ConA刺激的增殖以及ConA诱导的T母细胞产生IL2。同样，低剂量脂多糖（LPS）或CpG寡脱氧核苷酸1826刺激的B母细胞的增殖和IL6产生也被血小板显著抑制。有丝分裂原诱导的早期激活标志物CD69在T母细胞和B母细胞中的过表达均被血小板阻断。培养中的血小板还阻断了抗CD40/IL4或LPS/IL4处理诱导的B细胞中IgM和IgE的产生。这些观察结果为血小板在免疫区室中发挥更复杂作用的理论提供了新证据。应该做出更多努力来解决这种血小板-淋巴细胞相互作用在人和动物中是否具有任何生理意义的问题
