Kuse R, Burmeister H, Hausmann K
Blut. 1977 Sep 29;35(3):253-9. doi: 10.1007/BF00999467.
Platelet counts in platelet-rich plasma without hematocrit dependent correction were performed by following rapid and simple steps: 1. pre-dilution of 20 microliter of whole blood by an isotonic solution 1:25; 2. stabilized low-speed centrifugation with 55 g for 5 minutes; 3. final dilution 1 : 5000; 4. enumeration by use of a TOA platelet counter PL-100 which has been technically improved in comparison to similar machines. Erroneously high results were obtained after a too short or too low centrifugation. As reason for this artifical small pulses due to disturbances of the flow patterns around the aperture (so-called vortex-effect) can be assumed having been caused by large-volumed erythrocytes and leukocytes in the suspension. The routinely used procedure was reliable for all platelet ranges, especially in thrombocytopenias between 100 X 10(9)/l and 25 X 10(9)l. In lower ranges comparisons with visual counts are essential.
对未进行血细胞比容依赖性校正的富血小板血浆进行血小板计数，可按以下快速简单步骤进行：1. 用等渗溶液按1:25对20微升全血进行预稀释；2. 以55克进行低速稳定离心5分钟；3. 最终稀释1:5000；4. 使用经过技术改进的TOA血小板计数器PL-100进行计数，与类似机器相比，该计数器有所改进。离心时间过短或转速过低会导致错误的高结果。造成这种情况的原因可能是悬浮液中大量的红细胞和白细胞导致小孔周围流动模式受到干扰，从而产生人为的小脉冲（所谓的涡旋效应）。常规使用的程序对所有血小板范围都是可靠的，尤其是在血小板减少症患者血小板计数在100×10⁹/L至25×10⁹/L之间时。在较低计数范围内，与目视计数进行比较至关重要。
