Sandlow J I, Feng H L, Sandra A
Department of Urology, University of Iowa, Iowa City 52242-1089, USA.
Urology. 1997 Mar;49(3):494-500. doi: 10.1016/S0090-4295(96)00494-3.
To examine the localization and expression of the c-kit receptor protein in the testes of the mouse, rat, and human, and then compare these among the three species.
Testis tissue from all three species was obtained through biopsy or orchiectomy. Immunohistochemistry was used for the localization, using a monoclonal antibody to the c-kit receptor. The expression of the c-kit receptor protein was examined in the testes and sperm with Western blot analysis.
Localization was noted in the early spermatogenic cells, most likely type A spermatogonia, as well as in the acrosomal region of more mature germ cells, such as the round spermatids. The c-kit receptor was localized in analogous sites in all three species. The Western blot data revealed testicular expression of the c-kit receptor protein in all three species as well. Similar bands were recognized on the Western blots of all three species in testes at approximately 75 kDa and approximately 90 kDa, and sperm at approximately 90 kDa only.
The c-kit receptor protein is expressed in the early spermatogenic cells, as well as the later stages of spermatogenesis, specifically, the acrosomal granules of the round spermatids, and the acrosomal region of testicular spermatozoa, in the mouse, rat, and human. All three species exhibit similar expression of the c-kit receptor protein in both testis and sperm, although to a varying degree. We believe that these observations allow direct valid comparisons concerning the expression of the c-kit receptor to be made cautiously to the human condition from experimental data obtained from rodents.
检测c-kit受体蛋白在小鼠、大鼠和人类睾丸中的定位与表达，然后比较这三种物种之间的差异。
通过活检或睾丸切除术获取所有三种物种的睾丸组织。使用针对c-kit受体的单克隆抗体，采用免疫组织化学方法进行定位。通过蛋白质免疫印迹分析检测睾丸和精子中c-kit受体蛋白的表达。
在早期生精细胞中观察到定位，最可能是A型精原细胞，以及在更成熟生殖细胞的顶体区域，如圆形精子细胞。c-kit受体在所有三种物种的类似部位均有定位。蛋白质免疫印迹数据显示，所有三种物种的睾丸中均有c-kit受体蛋白表达。在所有三种物种睾丸的蛋白质免疫印迹上，约75 kDa和约90 kDa处均识别出相似条带，而在精子的蛋白质免疫印迹上仅在约90 kDa处识别出相似条带。
c-kit受体蛋白在小鼠、大鼠和人类的早期生精细胞以及精子发生的后期阶段均有表达，具体而言，在圆形精子细胞的顶体颗粒以及睾丸精子的顶体区域表达。所有三种物种在睾丸和精子中均表现出c-kit受体蛋白的相似表达，尽管程度有所不同。我们认为，这些观察结果使得能够根据从啮齿动物获得的实验数据，谨慎地对人类情况下c-kit受体的表达进行直接有效的比较。
