Gentry R T, Wade G N, Blaustein J D
Brain Res. 1977 Oct 21;135(1):135-46. doi: 10.1016/0006-8993(77)91057-5.
In streptozotocin-diabetic female rats acute (24 h) withdrawal of insulin significantly impairs both estradiol + progesterone-induced sexual receptivity and cell nuclear concentration of [3H]estradiol in hypothalamus, preoptic area, and pituitary gland. Omission of insulin treatment for the first 24 h of a 30-h or 54-h estradiol benzoate-conditioning period significantly reduced mean lordosis ratings of ovariectomized-diabetic rats. Insulin withdrawal at the time of progesterone treatment and behavioral testing did not diminish sexual receptivity. One-half or 2 h after an intravenous injection of [3H]estradiol-17beta diabetic rats without insulin exhibited reduced cell nuclear [3H]estradiol concentrations (2 h) and/or diminished cell nuclear/whole homogenate concentration ratios (0.5 and 2 h). Twenty-four hour insulin withdrawal affected neither whole tissue [3H]estradiol uptake nor hypothalamus-preoptic area cytoplasmic estrogen-receptor content. These results: (1) suggest that diminished estradiol binding by target tissue cell nuclei may contribute to the well-known reproductive failures of female diabetics, and (2) support the concept that estradiol acts at the level of brain cell nuclei to induce female sexual behavior.
在链脲佐菌素诱导的糖尿病雌性大鼠中，急性（24小时）停用胰岛素会显著损害雌二醇+孕酮诱导的性接受能力以及下丘脑、视前区和垂体中[3H]雌二醇的细胞核浓度。在30小时或54小时苯甲酸雌二醇预处理期的前24小时省略胰岛素治疗，会显著降低去卵巢糖尿病大鼠的平均脊柱前凸评分。在孕酮治疗和行为测试时停用胰岛素不会减弱性接受能力。静脉注射[3H]雌二醇-17β后半小时或2小时，未使用胰岛素的糖尿病大鼠的细胞核[3H]雌二醇浓度降低（2小时）和/或细胞核/全匀浆浓度比降低（半小时和2小时）。24小时停用胰岛素对全组织[3H]雌二醇摄取或下丘脑-视前区细胞质雌激素受体含量均无影响。这些结果：（1）表明靶组织细胞核雌二醇结合减少可能导致女性糖尿病患者众所周知的生殖功能障碍，（2）支持雌二醇在脑细胞核水平起作用以诱导雌性性行为的概念。
