Morimoto K, Sugimoto Y, Katsuyama M, Oida H, Tsuboi K, Kishi K, Kinoshita Y, Negishi M, Chiba T, Narumiya S, Ichikawa A
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Am J Physiol. 1997 Mar;272(3 Pt 1):G681-7. doi: 10.1152/ajpgi.1997.272.3.G681.
Regional and cellular distribution of mRNAs for prostaglandin E (PGE) receptor subtypes was investigated in the mouse gastrointestinal tract by in situ hybridization. Strong signals for EP1 transcripts were detected in cells of the muscularis mucosae layer, especially in the body of the stomach. Intense signals for EP3 transcripts were detected in neurons of the myenteric ganglia throughout the tract. Moderate EP3 mRNA expression was also observed in fundic gland epithelial cells, except for surface mucous cells in the stomach. Expression of EP4 mRNA was moderate in surface epithelial cells of the corpus and in glands from the surface to the base of the antrum. Strong EP4 signals were observed in the epithelium in the duodenum, jejunum, and ileum. In the ileum, signals were only observed in the upper part of the villi. However, no or weak signals for EP2 transcripts were detected. These findings suggest that PGE2 modulates various gastric or intestinal functions via at least three different PGE receptors.
通过原位杂交技术，对小鼠胃肠道中前列腺素E（PGE）受体亚型的mRNA进行了区域和细胞分布研究。在黏膜肌层细胞中检测到EP1转录本的强信号，尤其是在胃体部。在整个胃肠道的肌间神经节神经元中检测到EP3转录本的强烈信号。在胃底腺上皮细胞中也观察到中等程度的EP3 mRNA表达，但胃表面黏液细胞除外。EP4 mRNA在胃体表面上皮细胞以及从胃窦表面到基部的腺体中表达中等。在十二指肠、空肠和回肠的上皮中观察到强烈的EP4信号。在回肠中，信号仅在绒毛上部观察到。然而，未检测到EP2转录本的信号或信号较弱。这些发现表明，PGE2通过至少三种不同的PGE受体调节各种胃或肠功能。
