Jackson M L, Haines D M, Taylor S M, Misra V
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
J Vet Diagn Invest. 1996 Jan;8(1):25-30. doi: 10.1177/104063879600800105.
Clinicopathologic criteria were used to group 68 cats according to high, moderate, or low suspicion of having feline leukemia virus (FeLV)-related disease. Peripheral blood samples were tested for FeLV antigen by enzyme-linked immunosorbent assay (ELISA) and for FeLV DNA by polymerase chain reaction (PCR). There was no significant difference between ELISA and PCR results in the 68 cats. In the high-suspicion group, 46%(11/24) of cytopenic cats were test positive (ELISA and PCR) and 87% (13/15) with hemopoietic neoplasms were test-positive. Also within the high suspicion group, test-positive cats were 2.5 times more likely to die within the 1 year follow-up period than were test-negative (ELISA and PCR) cats. Among cats in the moderate-suspicion group, 15% (2/13) were test-positive, and none (0/16) of the cats in the low suspicion group was test positive. The relative risk of a positive test (ELISA and PCR) in the high suspicion group was 3.7 times that for the moderate-suspicion group and 22.8 times that for the low suspicion group. There was no significant difference in the relative risk of a positive test result between the moderate and low suspicion groups. The results indicate that FeLV detection by PCR can be adapted for diagnostic purposes using peripheral blood samples, however, results do not differ significantly from FeLV ELISA results. Also, a proportion of cats with a high suspicion of having FeLV-related cytopenia and hemopoietic tumors are negative for both circulating FeLV antigen and DNA. These cats may not have FeLV-related disease, or FeLV may exist in a disease-producing but nonreplicating form ultimately detectable by PCR in tissues other than peripheral blood.
采用临床病理标准，根据对猫白血病病毒（FeLV）相关疾病的高度、中度或低度怀疑，将68只猫进行分组。通过酶联免疫吸附测定（ELISA）检测外周血样本中的FeLV抗原，并通过聚合酶链反应（PCR）检测FeLV DNA。在这68只猫中，ELISA和PCR结果之间没有显著差异。在高度怀疑组中，血细胞减少的猫有46%（11/24）检测呈阳性（ELISA和PCR），造血肿瘤的猫有87%（13/15）检测呈阳性。同样在高度怀疑组中，检测呈阳性的猫在1年随访期内死亡的可能性是检测呈阴性（ELISA和PCR）猫的2.5倍。在中度怀疑组的猫中，15%（2/13）检测呈阳性，低度怀疑组的猫无一（0/16）检测呈阳性。高度怀疑组检测呈阳性（ELISA和PCR）的相对风险是中度怀疑组的3.7倍，是低度怀疑组的22.8倍。中度和低度怀疑组之间检测呈阳性结果的相对风险没有显著差异。结果表明，通过PCR检测FeLV可适用于使用外周血样本进行诊断，然而，结果与FeLV ELISA结果没有显著差异。此外，一部分高度怀疑患有FeLV相关血细胞减少症和造血肿瘤的猫，其循环FeLV抗原和DNA均为阴性。这些猫可能没有FeLV相关疾病，或者FeLV可能以一种致病但非复制的形式存在，最终可通过PCR在外周血以外的组织中检测到。
