[绿脓杆菌细胞成分的免疫学研究。V. 黏液及其组分的抗原分析]

[Immunologic study of the cellular components of bacillus pyocyaneus. V. Antigenic analysis of slime and its fractions].

作者信息

Zaĭdner I G, Stanislavskiĭ E S, Kolker I I, Grishina I A, Palkina N A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1979 Sep(9):41-5.

Abstract

Various slime fractions were obtained from newly isolated mucoid strains of P. aeruginosa by the method of ultrafiltration or differential centrifugation with subsequent gel chromatography. Purified slime was found to react with a broader spectrum of typing O sera than the corresponding cell wall lipopolysaccharides. Erythrocytic diagnostic preparations produced on the basis of slime antigens allowed to reveale the presence of circulating antibodies against P. aeruginosa. The slime components with molecular weight of 30,000--100,000 daltons and greater contained common antigenic determinants, and the slime components with molecular weight of 10,000--30,000 daltons contained both specific antigenic determinants and those also common to the high molecular components.

摘要

通过超滤或差速离心并随后进行凝胶色谱法，从新分离出的黏液型铜绿假单胞菌菌株中获得了各种黏液组分。发现纯化后的黏液比相应的细胞壁脂多糖能与更广泛的O型分型血清发生反应。基于黏液抗原制备的红细胞诊断制剂能够检测出抗铜绿假单胞菌循环抗体的存在。分子量为30,000 - 100,000道尔顿及以上的黏液成分含有共同的抗原决定簇，而分子量为10,000 - 30,000道尔顿的黏液成分既含有特异性抗原决定簇，也含有与高分子量成分共有的抗原决定簇。