Kolker I I, Stanislavskiĭ E S, Zarubina E K, Elagina E K, Kaliaev A V
Zh Mikrobiol Epidemiol Immunobiol. 1976(12):113-07.
The authors elaborated a method of obtaining a highly active lipopolysaccharide preparation from the cell membranes of the Pseudomonas aeruginosa whose sensitizing activity exceeded that of the polysaccharide isolated from the intact microbial cells almost 20 times. The optimal sensitizing dose of the preparation constituted 15 micron/ml. An erythrocytic diagnostic agent permitting to determine the antibodies in the sera of patients with the purulent-in flammatory diseases caused by Ps. aeruginosa was prepared on the basis of the purified lipo polysaccharide. A definite dependence of the hemagglutinin titres on the severity of the affection and the degree of the purulent-inflammatory process was revealed.
作者阐述了一种从铜绿假单胞菌细胞膜中获取高活性脂多糖制剂的方法，该制剂的致敏活性比从完整微生物细胞中分离出的多糖高出近20倍。该制剂的最佳致敏剂量为15微克/毫升。基于纯化的脂多糖制备了一种红细胞诊断剂，用于测定由铜绿假单胞菌引起的化脓性炎症疾病患者血清中的抗体。结果显示，血凝素滴度与病情严重程度及化脓性炎症过程的程度之间存在一定的相关性。
