Robinson J D, Risby D
Clin Chem. 1977 Nov;23(11):2085-8.
We describe a radioimmunoassay for the neuroleptic drug flupenthixol, suitable for routine monitoring of its concentration in blood. Antibodies for the assay were raised in a sheep against a 7-carboxyflupenthixol/ovalbumin conjugate. The resulting assay, with [3H] flupenthixol as the label, is capable of detecting 2.0 microgram of flupenthixol per liter, in a 100-microliter plasma sample. The antiserum shows no cross reactivity with tricyclic drugs and low interference from the major metabolites of flupenthixol. Concentrations in plasma after a single oral dose of flupenthixol have been followed in one volunteer. Peak values were reached after 3 h. Determinations of flupenthixol after fortnightly intramuscular depot injections of the sustained-release preparation, flupenthixol decanoate, showed the extent of fluctuations during this period.
我们描述了一种用于抗精神病药物氟哌噻吨的放射免疫分析方法，适用于对其血液浓度进行常规监测。该分析方法所用的抗体是通过将7-羧基氟哌噻吨与卵清蛋白偶联物免疫绵羊制备而成。最终得到的以[³H]氟哌噻吨为标记物的分析方法，能够在100微升血浆样本中检测到每升2.0微克的氟哌噻吨。该抗血清与三环类药物无交叉反应，且受氟哌噻吨主要代谢物的干扰较小。我们对一名志愿者单次口服氟哌噻吨后的血浆浓度进行了跟踪监测。3小时后达到峰值。对每两周进行一次长效制剂氟哌噻吨癸酸酯肌肉注射后氟哌噻吨的测定，显示了这段时间内的波动程度。
