Frigola A, Angeloni S, Cerqueti A R
Clin Chem. 1977 Nov;23(11):2103-6.
We describe a new method for determination of thrombin-clottable fibrinogen, which eliminates the systematic error caused by occlusion of other serum proteins in the fibrin clot and reduces the sensitivity to high concentrations of fibrin degradation products. Essentially, the method consists of densitometric quantitation of the fibrin band after a standard electrophoresis run of plasma, thrombin fixation of the fibrinogen, and removal of the non-clotted proteins by washing in saline. The procedure shows good precision and gives results that are accurate, significantly correlate with results for the classical thrombin clotting method (r = 0.92, P less than .001), and are not affected by fibrin degradation product concentrations up to 900 mg/liter. These characteristics make the method especially valuable in establishing fibrogen concentration in patients who are undergoing thrombolytic therapy.
我们描述了一种测定凝血酶可凝固纤维蛋白原的新方法，该方法消除了纤维蛋白凝块中其他血清蛋白阻塞所引起的系统误差，并降低了对高浓度纤维蛋白降解产物的敏感性。本质上，该方法包括对血浆进行标准电泳后对纤维蛋白带进行光密度定量、纤维蛋白原的凝血酶固定以及通过在盐水中洗涤去除未凝固的蛋白质。该程序显示出良好的精密度，给出的结果准确，与经典凝血酶凝血法的结果显著相关（r = 0.92，P小于0.001），并且不受高达900毫克/升的纤维蛋白降解产物浓度的影响。这些特性使得该方法在确定接受溶栓治疗患者的纤维蛋白原浓度方面特别有价值。
